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CRISPR

Overview of attention for book
Cover of 'CRISPR'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Investigating CRISPR RNA Biogenesis and Function Using RNA-seq.
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    Chapter 2 In Vitro Co-reconstitution of Cas Protein Complexes.
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    Chapter 3 Analysis of CRISPR Pre-crRNA Cleavage
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    Chapter 4 Annotation and Classification of CRISPR-Cas Systems.
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    Chapter 5 Computational Detection of CRISPR/crRNA Targets
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    Chapter 6 High-Throughput CRISPR Typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium.
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    Chapter 7 Spacer-Based Macroarrays for CRISPR Genotyping
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    Chapter 8 Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS).
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    Chapter 9 Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR.
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    Chapter 10 Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification of Target Interference Motifs in Escherichia coli
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    Chapter 11 CRISPR
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    Chapter 12 Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.
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    Chapter 13 Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids.
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    Chapter 14 Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition.
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    Chapter 15 Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function.
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    Chapter 16 Analysis of nuclease activity of cas1 proteins against complex DNA substrates.
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    Chapter 17 Characterizing Metal-Dependent Nucleases of CRISPR-Cas Prokaryotic Adaptive Immunity Systems.
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    Chapter 18 Cas3 Nuclease–Helicase Activity Assays
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    Chapter 19 Chemical and Enzymatic Footprint Analyses of R-Loop Formation by Cascade-crRNA Complex
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    Chapter 20 Creation and Analysis of a Virome: Using CRISPR Spacers.
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    Chapter 21 Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.
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    Chapter 22 Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
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    Chapter 23 Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi).
Attention for Chapter 22: Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

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1 blog
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9 X users
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1 Facebook page

Citations

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21 Dimensions

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140 Mendeley
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Chapter title
Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
Chapter number 22
Book title
CRISPR
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2687-9_22
Pubmed ID
Book ISBNs
978-1-4939-2686-2, 978-1-4939-2687-9
Authors

Gratz, Scott J, Harrison, Melissa M, Wildonger, Jill, O'Connor-Giles, Kate M, Scott J. Gratz, Melissa M. Harrison, Jill Wildonger, Kate M. O’Connor-Giles, Gratz, Scott J., Harrison, Melissa M., O’Connor-Giles, Kate M.

Editors

Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran

Abstract

The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.

X Demographics

X Demographics

The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 140 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Unknown 139 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 37 26%
Student > Ph. D. Student 35 25%
Student > Bachelor 16 11%
Student > Doctoral Student 9 6%
Student > Master 9 6%
Other 12 9%
Unknown 22 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 52 37%
Biochemistry, Genetics and Molecular Biology 51 36%
Neuroscience 7 5%
Medicine and Dentistry 2 1%
Chemical Engineering 1 <1%
Other 4 3%
Unknown 23 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2016.
All research outputs
#2,547,155
of 23,577,654 outputs
Outputs from Methods in molecular biology
#464
of 13,410 outputs
Outputs of similar age
#37,008
of 356,631 outputs
Outputs of similar age from Methods in molecular biology
#28
of 1,000 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,631 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 1,000 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.