Chapter title |
Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
|
---|---|
Chapter number | 22 |
Book title |
CRISPR
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2687-9_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2686-2, 978-1-4939-2687-9
|
Authors |
Gratz, Scott J, Harrison, Melissa M, Wildonger, Jill, O'Connor-Giles, Kate M, Scott J. Gratz, Melissa M. Harrison, Jill Wildonger, Kate M. O’Connor-Giles, Gratz, Scott J., Harrison, Melissa M., O’Connor-Giles, Kate M. |
Editors |
Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran |
Abstract |
The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach. |
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Mendeley readers
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