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CRISPR

Overview of attention for book
Cover of 'CRISPR'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Investigating CRISPR RNA Biogenesis and Function Using RNA-seq.
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    Chapter 2 In Vitro Co-reconstitution of Cas Protein Complexes.
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    Chapter 3 Analysis of CRISPR Pre-crRNA Cleavage
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    Chapter 4 Annotation and Classification of CRISPR-Cas Systems.
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    Chapter 5 Computational Detection of CRISPR/crRNA Targets
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    Chapter 6 High-Throughput CRISPR Typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium.
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    Chapter 7 Spacer-Based Macroarrays for CRISPR Genotyping
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    Chapter 8 Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS).
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    Chapter 9 Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR.
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    Chapter 10 Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification of Target Interference Motifs in Escherichia coli
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    Chapter 11 CRISPR
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    Chapter 12 Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.
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    Chapter 13 Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids.
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    Chapter 14 Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition.
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    Chapter 15 Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function.
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    Chapter 16 Analysis of nuclease activity of cas1 proteins against complex DNA substrates.
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    Chapter 17 Characterizing Metal-Dependent Nucleases of CRISPR-Cas Prokaryotic Adaptive Immunity Systems.
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    Chapter 18 Cas3 Nuclease–Helicase Activity Assays
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    Chapter 19 Chemical and Enzymatic Footprint Analyses of R-Loop Formation by Cascade-crRNA Complex
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    Chapter 20 Creation and Analysis of a Virome: Using CRISPR Spacers.
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    Chapter 21 Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.
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    Chapter 22 Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
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    Chapter 23 Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi).
Attention for Chapter 9: Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR.
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Chapter title
Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR.
Chapter number 9
Book title
CRISPR
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2687-9_9
Pubmed ID
25981471
Book ISBNs
978-1-4939-2686-2, 978-1-4939-2687-9
Authors

Strotksaya, Alexandra, Semenova, Ekaterina, Savitskaya, Ekaterina, Severinov, Konstantin, Alexandra Strotksaya, Ekaterina Semenova, Ekaterina Savitskaya, Konstantin Severinov

Editors

Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran

Abstract

In Escherichia coli, acquisition of new spacers in the course of CRISPR-Cas adaptation is dramatically stimulated by preexisting partial matches between a bacterial CRISPR cassette spacer and a protospacer sequence in the DNA of the infecting bacteriophage or plasmid. This phenomenon, which we refer to as "priming," can be used for very simple and rapid construction of multiple E. coli strains capable of targeting, through CRISPR interference, any phage or plasmid of interest. Availability of such strains should allow rapid progress in the analysis of CRISPR-Cas system function against diverse mobile genetic elements.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 27%
Student > Master 3 14%
Student > Ph. D. Student 3 14%
Professor > Associate Professor 2 9%
Other 1 5%
Other 2 9%
Unknown 5 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 36%
Agricultural and Biological Sciences 7 32%
Veterinary Science and Veterinary Medicine 1 5%
Medicine and Dentistry 1 5%
Unknown 5 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2015.
All research outputs
#18,410,971
of 22,805,349 outputs
Outputs from Methods in molecular biology
#7,906
of 13,120 outputs
Outputs of similar age
#255,827
of 353,075 outputs
Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,120 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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