| Chapter title |
Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.
|
|---|---|
| Chapter number | 21 |
| Book title |
CRISPR
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2687-9_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2686-2, 978-1-4939-2687-9
|
| Authors |
Hwang, Woong Y, Fu, Yanfang, Reyon, Deepak, Gonzales, Andrew P W, Joung, J Keith, Yeh, Jing-Ruey Joanna, Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Andrew P. W. Gonzales, J. Keith Joung, Jing-Ruey Joanna Yeh, Hwang, Woong Y., Gonzales, Andrew P. W., Joung, J. Keith |
| Editors |
Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran |
| Abstract |
In recent years, the zebrafish has become a critical contributor to various areas of biomedical research, advancing our fundamental understanding of biomedicine and helping discover candidate therapeutics for human diseases. Nevertheless, to further extend the power of this important model organism requires a robust and simple-to-use genome editing platform that will enable targeted gene knockouts and introduction of specific mutations identified in human diseases into the zebrafish genome. We describe here protocols for creating insertion or deletion (indel) mutations or precise sequence modifications in zebrafish genes using customizable CRISPR-Cas9 RNA-guided nucleases (RGNs). These methods can be easily implemented in any lab and may also potentially be extended for use in other organisms. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 3% |
| Unknown | 39 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 23% |
| Professor > Associate Professor | 5 | 13% |
| Student > Master | 5 | 13% |
| Student > Ph. D. Student | 4 | 10% |
| Student > Bachelor | 3 | 8% |
| Other | 4 | 10% |
| Unknown | 10 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 40% |
| Agricultural and Biological Sciences | 10 | 25% |
| Medicine and Dentistry | 3 | 8% |
| Arts and Humanities | 1 | 3% |
| Unknown | 10 | 25% |