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CRISPR

Overview of attention for book
Cover of 'CRISPR'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Investigating CRISPR RNA Biogenesis and Function Using RNA-seq.
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    Chapter 2 In Vitro Co-reconstitution of Cas Protein Complexes.
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    Chapter 3 Analysis of CRISPR Pre-crRNA Cleavage
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    Chapter 4 Annotation and Classification of CRISPR-Cas Systems.
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    Chapter 5 Computational Detection of CRISPR/crRNA Targets
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    Chapter 6 High-Throughput CRISPR Typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium.
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    Chapter 7 Spacer-Based Macroarrays for CRISPR Genotyping
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    Chapter 8 Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS).
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    Chapter 9 Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR.
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    Chapter 10 Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification of Target Interference Motifs in Escherichia coli
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    Chapter 11 CRISPR
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    Chapter 12 Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.
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    Chapter 13 Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids.
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    Chapter 14 Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition.
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    Chapter 15 Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function.
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    Chapter 16 Analysis of nuclease activity of cas1 proteins against complex DNA substrates.
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    Chapter 17 Characterizing Metal-Dependent Nucleases of CRISPR-Cas Prokaryotic Adaptive Immunity Systems.
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    Chapter 18 Cas3 Nuclease–Helicase Activity Assays
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    Chapter 19 Chemical and Enzymatic Footprint Analyses of R-Loop Formation by Cascade-crRNA Complex
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    Chapter 20 Creation and Analysis of a Virome: Using CRISPR Spacers.
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    Chapter 21 Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.
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    Chapter 22 Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
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    Chapter 23 Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi).
Attention for Chapter 12: Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.
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Chapter title
Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.
Chapter number 12
Book title
CRISPR
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2687-9_12
Pubmed ID
25981474
Book ISBNs
978-1-4939-2686-2, 978-1-4939-2687-9
Authors

Zhang, Jing, White, Malcolm F, Jing Zhang, Malcolm F. White

Editors

Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran

Abstract

Protein purification is an important technique that allows us to characterize the structural and biochemical properties of either an individual protein or a multi-protein complex. However, expression and purification of one subunit of a complex in the absence of its binding partners has often proven difficult to achieve due to the issues such as instability and mis-folding. This is the case for the components of the CRISPR-Cas interference complexes, which degrade invading nucleic acids in a sequence homology-dependent manner in many prokaryotic species. Here, we describe the expression of a tandem-tagged subunit of the Type III-B (CMR) complex in Sulfolobus solfataricus and subsequent isolation and purification of the whole complex by affinity purification of the tagged subunit.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 29%
Student > Ph. D. Student 1 14%
Other 1 14%
Student > Master 1 14%
Professor > Associate Professor 1 14%
Other 0 0%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 57%
Agricultural and Biological Sciences 2 29%
Unknown 1 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2016.
All research outputs
#20,305,223
of 22,844,985 outputs
Outputs from Methods in molecular biology
#9,917
of 13,127 outputs
Outputs of similar age
#295,947
of 353,225 outputs
Outputs of similar age from Methods in molecular biology
#636
of 997 outputs
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So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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