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Mendeley readers
| Chapter title |
CRISPR
|
|---|---|
| Chapter number | 11 |
| Book title |
CRISPR
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2687-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2686-2, 978-1-4939-2687-9
|
| Authors |
Künne, Tim, Westra, Edze R, Brouns, Stan J J, Westra, Edze R., Brouns, Stan J. J., Tim Künne, Edze R. Westra, Stan J. J. Brouns |
| Editors |
Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran |
| Abstract |
The Electrophoretic Mobility Shift Assay is a straightforward and inexpensive method for the determination and quantification of protein-nucleic acid interactions. It relies on the different mobility of free and protein-bound nucleic acid in a gel matrix during electrophoresis. Nucleic acid affinities of crRNA-Cas complexes can be quantified by calculating the dissociation constant (K d). Here, we describe how two types of EMSA assays are performed using the Cascade ribonucleoprotein complex from Escherichia coli as an example. |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 26% |
| Student > Master | 5 | 19% |
| Researcher | 5 | 19% |
| Student > Bachelor | 3 | 11% |
| Professor > Associate Professor | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 41% |
| Agricultural and Biological Sciences | 10 | 37% |
| Immunology and Microbiology | 1 | 4% |
| Chemistry | 1 | 4% |
| Unknown | 4 | 15% |