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The Tobacco mosaic virus Movement Protein Associates with but Does Not Integrate into Biological Membranes

Overview of attention for article published in Journal of Virology, March 2014
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

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Title
The Tobacco mosaic virus Movement Protein Associates with but Does Not Integrate into Biological Membranes
Published in
Journal of Virology, March 2014
DOI 10.1128/jvi.03648-13
Pubmed ID
Authors

Ana Peiró, Luis Martínez-Gil, Silvia Tamborero, Vicente Pallás, Jesús A. Sánchez-Navarro, Ismael Mingarro

Abstract

Plant positive-strand RNA viruses require association with plant cell endomembranes for viral translation and replication, as well as for intra- and intercellular movement of the viral progeny. The membrane association and RNA binding of the Tobacco mosaic virus (TMV) movement protein (MP) are vital for orchestrating the macromolecular network required for virus movement. A previously proposed topological model suggests that TMV MP is an integral membrane protein with two putative α-helical transmembrane (TM) segments. Here we tested this model using an experimental system that measured the efficiency with which natural polypeptide segments were inserted into the ER membrane under conditions approximating the in vivo situation, as well as in planta. Our results demonstrated that the two hydrophobic regions (HRs) of TMV MP do not span biological membranes. We further found that mutations to alter the hydrophobicity of the first HR modified membrane association and precluded virus movement. We propose a topological model in which the TMV MP HRs intimately associate with the cellular membranes, allowing maximum exposure of the hydrophilic domains of the MP to the cytoplasmic cellular components. Importance: To facilitate plant viral infection and spread, viruses encode one or more movement proteins (MPs) that interact with ER membranes. The present work investigated the membrane association of the 30K MP of Tobacco mosaic virus (TMV), and the results challenge the previous topological model, which predicted that the TMV MP behaves as an integral membrane protein. The current data provide greatly needed clarification of the topological model and provide substantial evidence that TMV MP is membrane associated only at the cytoplasmic face of the membrane and that neither of its domains is integrated into the membrane or translocated into the lumen. Understanding the topology of MPs in the ER is vital for understanding the role of the ER in plant virus transport and for predicting interactions with host factors that mediate resistance to plant viruses.

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Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 2%
Argentina 1 2%
Unknown 63 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 20%
Student > Ph. D. Student 11 17%
Student > Bachelor 10 15%
Professor 7 11%
Student > Master 6 9%
Other 9 14%
Unknown 9 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 51%
Biochemistry, Genetics and Molecular Biology 15 23%
Chemistry 2 3%
Computer Science 1 2%
Arts and Humanities 1 2%
Other 2 3%
Unknown 11 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2014.
All research outputs
#2,524,227
of 20,421,224 outputs
Outputs from Journal of Virology
#1,914
of 23,625 outputs
Outputs of similar age
#27,067
of 199,866 outputs
Outputs of similar age from Journal of Virology
#24
of 165 outputs
Altmetric has tracked 20,421,224 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 23,625 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 199,866 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 165 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.