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Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714

Overview of attention for article published in AMB Express, July 2017
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Title
Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714
Published in
AMB Express, July 2017
DOI 10.1186/s13568-017-0443-9
Pubmed ID
Authors

Donya Kamravamanesh, Stefan Pflügl, Winfried Nischkauer, Andreas Limbeck, Maximilian Lackner, Christoph Herwig

Abstract

Poly-β-hydroxybutyrate (PHB) production from CO2 has the potential to reduce the production cost of this biodegradable polyesters, and also to make the material more sustainable compared to utilization of sugar feedstocks. In this study the unicellular cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential organism for production of PHB. Synechocystis sp. PCC 6714 was studied under various cultivation conditions and nutritional limitations. Combined effects of nitrogen and phosphorus deficiency led to highest PHB accumulation under photoautotrophic conditions. Multivariate experimental design and quantitative bioprocess development methodologies were used to identify the key cultivation parameters for PHB accumulation. Biomass growth and PHB accumulation were studied under controlled defined conditions in a lab-scale photobioreactor. Specific growth rates were fourfold higher in photobioreactor experiments when cultivation conditions were controlled. After 14 days of cultivation in nitrogen and phosphorus, limited media intracellular PHB levels reached up to 16.4% from CO2. The highest volumetric production rate of PHB was 59 ± 6 mg L(-1) day(-1). Scanning electron microscopy of isolated PHB granules of Synechocystis sp. PCC 6714 cultivated under nitrogen and phosphorus limitations showed an average diameter of 0.7 µm. The results of this study might contribute towards a better understanding of photoautotrophic PHB production from cyanobacteria.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 125 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 20%
Student > Master 18 14%
Student > Bachelor 17 14%
Researcher 14 11%
Student > Doctoral Student 4 3%
Other 12 10%
Unknown 35 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 23 18%
Agricultural and Biological Sciences 17 14%
Environmental Science 9 7%
Engineering 8 6%
Chemical Engineering 8 6%
Other 12 10%
Unknown 48 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2017.
All research outputs
#15,467,628
of 22,985,065 outputs
Outputs from AMB Express
#446
of 1,239 outputs
Outputs of similar age
#197,375
of 313,520 outputs
Outputs of similar age from AMB Express
#32
of 61 outputs
Altmetric has tracked 22,985,065 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,239 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,520 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 61 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.