Chapter title |
High-Density Serum/Plasma Reverse Phase Protein Arrays
|
---|---|
Chapter number | 18 |
Book title |
Serum/Plasma Proteomics
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7057-5_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7056-8, 978-1-4939-7057-5
|
Authors |
Cecilia Hellström, Tea Dodig-Crnković, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, Ronald Sjöberg |
Editors |
David W. Greening, Richard J. Simpson |
Abstract |
In-depth exploration and characterization of human serum and plasma proteomes is an attractive strategy for the identification of potential prognostic or diagnostic biomarkers. The possibility of analyzing larger numbers of samples in a high-throughput fashion has markedly increased with affinity-based microarrays, thus providing higher statistical power to these biomarker studies. Here, we describe a protocol for high-density serum and plasma reverse phase protein arrays (RPPAs). We demonstrate how a biobank of 12,392 samples was immobilized and analyzed on a single microarray slide, allowing high-quality profiling of abundant target proteins across all samples in one assay. |
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