| Chapter title |
Antibody Colocalization Microarray for Cross-Reactivity-Free Multiplexed Protein Analysis
|
|---|---|
| Chapter number | 19 |
| Book title |
Serum/Plasma Proteomics
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7057-5_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7056-8, 978-1-4939-7057-5
|
| Authors |
Véronique Laforte, Pik-Shan Lo, Huiyan Li, David Juncker, Laforte, Véronique, Lo, Pik-Shan, Li, Huiyan, Juncker, David |
| Editors |
David W. Greening, Richard J. Simpson |
| Abstract |
Measuring many proteins at once is of great importance to the idea of personalized medicine, in order to get a snapshot of a person's health status. We describe the antibody colocalization microarray (ACM), a variant of antibody microarrays which avoids reagent-induced cross-reactivity by printing individual detection antibodies atop their corresponding capture antibodies. We discuss experimental parameters that are critical for the success of ACM experiments, namely, the printing positional accuracy needed for the two printing rounds and the need for protecting dried spots during the second printing round. Using small sample volumes (less than 30 μL) and small quantities of reagents, up to 108 different targets can be measured in hundreds of samples with great specificity and sensitivity. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 20% |
| Student > Postgraduate | 1 | 20% |
| Other | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Medicine and Dentistry | 1 | 20% |
| Engineering | 1 | 20% |
| Unknown | 1 | 20% |