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Cell Microencapsulation

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Cover of 'Cell Microencapsulation'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Cell Microencapsulation
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    Chapter 2 Applications of Cell Microencapsulation
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    Chapter 3 Cell Microencapsulation: Dripping Methods
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    Chapter 4 Field Effect Microparticle Generation for Cell Microencapsulation
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    Chapter 5 Microfluidic Approach to Cell Microencapsulation
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    Chapter 6 Polymeric Materials for Cell Microencapsulation
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    Chapter 7 Polymeric Materials for Perm-Selective Coating of Alginate Microbeads
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    Chapter 8 Determination of the Mechanical Strength of Microcapsules
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    Chapter 9 The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation
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    Chapter 10 Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems
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    Chapter 11 Noninvasive Tracking of Alginate-Microencapsulated Cells
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    Chapter 12 Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation
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    Chapter 13 A Method of Porcine Pancreatic Islet Isolation for Microencapsulation
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    Chapter 14 Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation
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    Chapter 15 Preparation and Characterization of Alginate–Chitosan Microcapsule for Hepatocyte Culture
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    Chapter 16 Use of Flow Focusing Technique for Microencapsulation of Myoblasts
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    Chapter 17 Alginate Microbeads for Cell and Protein Delivery
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    Chapter 18 Compartmentalization of Two Cell Types in Multilayered Alginate Microcapsules
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    Chapter 19 Primary Choroid Plexus Tissue for Use in Cellular Therapy
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    Chapter 20 Cell Microencapsulation
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    Chapter 21 Microencapsulated Cells for Cancer Therapy
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    Chapter 22 Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic Application
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    Chapter 23 Microencapsulation of Islets for the Treatment of Type 1 Diabetes Mellitus (T1D)
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    Chapter 24 Cell Microencapsulation
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    Chapter 25 Microencapsulation in Clinical Islet Xenotransplantation
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    Chapter 26 Methods for Microencapsulated Porcine Islet Production
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    Chapter 27 Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism
Attention for Chapter 7: Polymeric Materials for Perm-Selective Coating of Alginate Microbeads
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Chapter title
Polymeric Materials for Perm-Selective Coating of Alginate Microbeads
Chapter number 7
Book title
Cell Microencapsulation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6364-5_7
Pubmed ID
Book ISBNs
978-1-4939-6362-1, 978-1-4939-6364-5
Authors

William F. Kendall Jr. M.D., Emmanuel C. Opara, William F. Kendall

Editors

Emmanuel C. Opara

Abstract

Application of microencapsulation to the immunoisolation of pancreatic islets holds promise for expanding the use of islet transplantation as a treatment option for Type 1 diabetes. It is generally believed that successful development of a reliable methodology will ideally allow for transplantation of pancreatic islets that are protected from the immune system, thereby obviating the need for the use of immunosuppressive drugs and their attendant side effects. In addition, this technology has the potential to expand the donor pool as islets from nonhuman donors could be used as xenografts in human patients. The complex polysaccharide, alginate, has been the most widely used polymer for microencapsulation of islets. However, it is known that alginate lacks appreciable permselectivity to confer immunoisolation of encapsulated islets, thus necessitating the routine permselective coating of alginate microbeads with polymers of amino acids, mainly, poly-L-lysine (PLL) and poly-L-ornithine (PLO). The protocol described in this chapter outlines the steps we have used in our studies on perm-selective coating of alginate microbeads for islet transplantation.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 33%
Unknown 2 67%
Readers by discipline Count As %
Unspecified 1 33%
Unknown 2 67%