Chapter title |
Noninvasive Tracking of Alginate-Microencapsulated Cells
|
---|---|
Chapter number | 11 |
Book title |
Cell Microencapsulation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6364-5_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6362-1, 978-1-4939-6364-5
|
Authors |
Genaro A. Paredes-Juarez, Brad P. Barnett, Jeff W. M. Bulte |
Editors |
Emmanuel C. Opara |
Abstract |
This chapter presents a description of standardized techniques used routinely in our laboratory to encapsulate different cell types using the alginate-PLL-alginate immunoisolation system. Given the importance of noninvasive tracking of encapsulated cell transplants, we present a detailed guidance to achieve maximum efficiency and functionality of the capsule preparations for optimal tracking posttransplantation. The provided protocols cover tracking of encapsulated cells using magnetic resonance (MR), X-ray, computed tomography (CT), and ultrasound (US) imaging. Practical suggestions to optimize each method with specific references to recommended suppliers are included. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 18% |
Student > Bachelor | 2 | 18% |
Professor | 1 | 9% |
Researcher | 1 | 9% |
Unknown | 5 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 27% |
Engineering | 1 | 9% |
Unknown | 7 | 64% |