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Cell Microencapsulation

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Cover of 'Cell Microencapsulation'

Table of Contents

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    Book Overview
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    Chapter 1 Cell Microencapsulation
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    Chapter 2 Applications of Cell Microencapsulation
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    Chapter 3 Cell Microencapsulation: Dripping Methods
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    Chapter 4 Field Effect Microparticle Generation for Cell Microencapsulation
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    Chapter 5 Microfluidic Approach to Cell Microencapsulation
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    Chapter 6 Polymeric Materials for Cell Microencapsulation
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    Chapter 7 Polymeric Materials for Perm-Selective Coating of Alginate Microbeads
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    Chapter 8 Determination of the Mechanical Strength of Microcapsules
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    Chapter 9 The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation
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    Chapter 10 Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems
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    Chapter 11 Noninvasive Tracking of Alginate-Microencapsulated Cells
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    Chapter 12 Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation
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    Chapter 13 A Method of Porcine Pancreatic Islet Isolation for Microencapsulation
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    Chapter 14 Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation
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    Chapter 15 Preparation and Characterization of Alginate–Chitosan Microcapsule for Hepatocyte Culture
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    Chapter 16 Use of Flow Focusing Technique for Microencapsulation of Myoblasts
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    Chapter 17 Alginate Microbeads for Cell and Protein Delivery
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    Chapter 18 Compartmentalization of Two Cell Types in Multilayered Alginate Microcapsules
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    Chapter 19 Primary Choroid Plexus Tissue for Use in Cellular Therapy
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    Chapter 20 Cell Microencapsulation
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    Chapter 21 Microencapsulated Cells for Cancer Therapy
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    Chapter 22 Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic Application
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    Chapter 23 Microencapsulation of Islets for the Treatment of Type 1 Diabetes Mellitus (T1D)
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    Chapter 24 Cell Microencapsulation
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    Chapter 25 Microencapsulation in Clinical Islet Xenotransplantation
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    Chapter 26 Methods for Microencapsulated Porcine Islet Production
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    Chapter 27 Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism
Attention for Chapter 13: A Method of Porcine Pancreatic Islet Isolation for Microencapsulation
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Chapter title
A Method of Porcine Pancreatic Islet Isolation for Microencapsulation
Chapter number 13
Book title
Cell Microencapsulation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6364-5_13
Pubmed ID
Book ISBNs
978-1-4939-6362-1, 978-1-4939-6364-5
Authors

William F. Kendall Jr. MD, Emmanuel C. Opara, William F. Kendall

Editors

Emmanuel C. Opara

Abstract

Since the discovery of insulin by Banting and Best in 1921, the prognosis and treatment options for individuals with diabetes have improved. The development of various insulin types, various oral agents, and insulin pumps have improved the available medical options for individuals afflicted with diabetes. The current need for frequent blood glucose monitoring imposed by multiple daily insulin injections, result in significant life-style challenges for in individuals afflicted with Type 1 diabetes (T1D). In contrast the use of surgical interventions, such as whole organ pancreas transplantation (PT) requires less-intensive glucose monitoring while the organ is viable. Also, isolated human pancreatic islet transplantation (IT) holds similar promise as PT; however, the limited availability of human pancreata exacerbated by, the need for multiple pancreata per individual IT recipient, and issues with prolonged viability, still hamper widespread successful, and routine use of IT. The use of porcine pancreata holds promise as a viable alternative to human pancreas to significantly increase the volume of islets available to meet the needs of millions of patients afflicted with T1D. This chapter outlines our protocol utilized to reliably isolate and microencapsulate porcine islets.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 20%
Student > Bachelor 1 20%
Student > Postgraduate 1 20%
Other 1 20%
Student > Doctoral Student 1 20%
Other 0 0%
Readers by discipline Count As %
Medicine and Dentistry 2 40%
Pharmacology, Toxicology and Pharmaceutical Science 1 20%
Unspecified 1 20%
Engineering 1 20%