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Cell Microencapsulation

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Cover of 'Cell Microencapsulation'

Table of Contents

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    Book Overview
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    Chapter 1 Cell Microencapsulation
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    Chapter 2 Applications of Cell Microencapsulation
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    Chapter 3 Cell Microencapsulation: Dripping Methods
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    Chapter 4 Field Effect Microparticle Generation for Cell Microencapsulation
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    Chapter 5 Microfluidic Approach to Cell Microencapsulation
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    Chapter 6 Polymeric Materials for Cell Microencapsulation
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    Chapter 7 Polymeric Materials for Perm-Selective Coating of Alginate Microbeads
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    Chapter 8 Determination of the Mechanical Strength of Microcapsules
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    Chapter 9 The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation
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    Chapter 10 Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems
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    Chapter 11 Noninvasive Tracking of Alginate-Microencapsulated Cells
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    Chapter 12 Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation
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    Chapter 13 A Method of Porcine Pancreatic Islet Isolation for Microencapsulation
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    Chapter 14 Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation
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    Chapter 15 Preparation and Characterization of Alginate–Chitosan Microcapsule for Hepatocyte Culture
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    Chapter 16 Use of Flow Focusing Technique for Microencapsulation of Myoblasts
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    Chapter 17 Alginate Microbeads for Cell and Protein Delivery
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    Chapter 18 Compartmentalization of Two Cell Types in Multilayered Alginate Microcapsules
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    Chapter 19 Primary Choroid Plexus Tissue for Use in Cellular Therapy
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    Chapter 20 Cell Microencapsulation
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    Chapter 21 Microencapsulated Cells for Cancer Therapy
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    Chapter 22 Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic Application
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    Chapter 23 Microencapsulation of Islets for the Treatment of Type 1 Diabetes Mellitus (T1D)
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    Chapter 24 Cell Microencapsulation
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    Chapter 25 Microencapsulation in Clinical Islet Xenotransplantation
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    Chapter 26 Methods for Microencapsulated Porcine Islet Production
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    Chapter 27 Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism
Attention for Chapter 18: Compartmentalization of Two Cell Types in Multilayered Alginate Microcapsules
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Chapter title
Compartmentalization of Two Cell Types in Multilayered Alginate Microcapsules
Chapter number 18
Book title
Cell Microencapsulation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6364-5_18
Pubmed ID
Book ISBNs
978-1-4939-6362-1, 978-1-4939-6364-5
Authors

Sivanandane Sittadjody, Justin M. Saul, Emmanuel C. Opara

Editors

Emmanuel C. Opara

Abstract

Two-dimensional (2D) culture systems do not represent the native microenvironment of the cells which is known to be three dimensional (3D), and surrounded by other cells from all directions. There exist interactions with other cell types in the same vicinity and this also cannot be replicated in a 2D culture. To study the cell-cell interactions between two or more cell types and their biological functions, a few 3D models have been used by different investigators. We have designed a 3D model to investigate the cell-cell interactions between various types of ovarian cells. The same model was also used to study the interactions between prostate cancer epithelial cells and stromal cells. This model uses hydrogel as the anchor matrix to fabricate the constructs and microencapsulation techniques to design multilayered microcapsules. In these multilayer microcapsules the different types of cells are compartmentalized by a sequential encapsulation process. In this chapter, we provide the protocol to compartmentalize two cell types in the same multilayer microcapsules. Although this chapter describes the fabrication of multilayer microcapsules with ovarian cells, the same approach could be applied to other multi-cell tissue-engineered constructs that require cell-cell interactions.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Student > Doctoral Student 2 25%
Student > Bachelor 2 25%
Student > Master 1 13%
Unknown 1 13%
Readers by discipline Count As %
Medicine and Dentistry 2 25%
Biochemistry, Genetics and Molecular Biology 1 13%
Veterinary Science and Veterinary Medicine 1 13%
Engineering 1 13%
Unknown 3 38%