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In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta

Overview of attention for article published in BMC Complementary and Alternative Medicine, June 2017
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Title
In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta
Published in
BMC Complementary and Alternative Medicine, June 2017
DOI 10.1186/s12906-017-1852-7
Pubmed ID
Authors

Faisal S. Alagrafi, Abdullah O. Alawad, Nael M. Abutaha, Fahd A. Nasr, Othman A. Alhazzaa, Sultan N. Alharbi, Mohammad N. Alkhrayef, Mohamed Hammad, Ziyad A. Alhamdan, Abdullah D. Alenazi, Mohammad A. Wadaan

Abstract

Rhazya stricta Decne. is a medicinal plant that is widespread in Saudi Arabia and desert areas of the Arabian Peninsula. Its extract contains alkaloids, tannins, and flavonoids that are involved in different biological activities. The study aim was to evaluate the effects of Rhazya stricta plant extracts on the proliferation and differentiation of NTERA-2 (NT2) pluripotent embryonal carcinoma cells. Soxhlet extraction was carried out using different solvents to extract stems, leaves and fruit parts of this plant. Cytotoxicity was evaluated by an MTS cell viability assay. The ability of the plant extract to induce cell differentiation was examined phenotypically using an inverted light microscope. The expression of pluripotency markers was investigated by reverse transcriptase polymerase chain reaction (RT-PCR) and immunocytochemistry. Phytochemical screening of chloroform stem extracts was carried out and a chromatographic fingerprint was generated using gas chromatography - mass spectrometry (GC-MS). Chloroform stem extract induced differentiation of NT2 cells at 5 μg/ml, and the differentiated cells exhibited neurite formation. Following induction of differentiation, there was significant down-regulation of the pluripotency marker genes Oct4 and Sox2. In addition, the surface antigen pluripotency marker, TRA-1-60, was strongly down-regulated. Phytochemical analysis of the extract showed the presence of alkaloids and saponins. The chromatogram revealed the presence of fifteen compounds with different retention times. Our results demonstrate for the first time that chloroform stem extract of R. stricta can induce neuronal differentiation of stem cells at an early stage and may contain potential therapeutic agent that can be used in neurodegenerative diseases.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 33%
Unspecified 1 8%
Lecturer 1 8%
Lecturer > Senior Lecturer 1 8%
Student > Ph. D. Student 1 8%
Other 2 17%
Unknown 2 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 33%
Biochemistry, Genetics and Molecular Biology 3 25%
Nursing and Health Professions 1 8%
Unspecified 1 8%
Chemistry 1 8%
Other 0 0%
Unknown 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2017.
All research outputs
#9,156,534
of 11,438,239 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,656
of 2,384 outputs
Outputs of similar age
#189,134
of 259,672 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#18
of 24 outputs
Altmetric has tracked 11,438,239 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,384 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 259,672 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.