Title |
Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen
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Published in |
Brazilian Journal of Microbiology, June 2017
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DOI | 10.1016/j.bjm.2017.03.006 |
Pubmed ID | |
Authors |
Yun-Hee Song, Kyung-Tai Lee, Jin-Young Baek, Min-Ju Kim, Mi-Ra Kwon, Young-Joo Kim, Mi-Rim Park, Haesu Ko, Jin-Sung Lee, Keun-Sung Kim |
Abstract |
The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase family 5 cellulases. The novel glycosyl hydrolase family 5 cellulase gene was overexpressed in Escherichia coli. Substrate specificity analysis revealed that this recombinant glycosyl hydrolase family 5 cellulase functions as an endo-β-1,4-glucanase. The recombinant KG35 endo-β-1,4-glucanase showed optimal activity within the range of 30-50°C at a pH of 6-7. The thermostability was retained and the pH was stable in the range of 30-50°C at a pH of 5-7. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 49 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 18% |
Student > Bachelor | 8 | 16% |
Student > Master | 4 | 8% |
Researcher | 4 | 8% |
Lecturer | 2 | 4% |
Other | 6 | 12% |
Unknown | 16 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 11 | 22% |
Agricultural and Biological Sciences | 8 | 16% |
Immunology and Microbiology | 3 | 6% |
Chemistry | 2 | 4% |
Environmental Science | 1 | 2% |
Other | 1 | 2% |
Unknown | 23 | 47% |