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Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen

Overview of attention for article published in Brazilian Journal of Microbiology, October 2017
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4 tweeters

Citations

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17 Dimensions

Readers on

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48 Mendeley

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Title
Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen
Published in
Brazilian Journal of Microbiology, October 2017
DOI 10.1016/j.bjm.2017.03.006
Pubmed ID
28689814
Authors

Yun-Hee Song, Kyung-Tai Lee, Jin-Young Baek, Min-Ju Kim, Mi-Ra Kwon, Young-Joo Kim, Mi-Rim Park, Haesu Ko, Jin-Sung Lee, Keun-Sung Kim

Abstract

The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase family 5 cellulases. The novel glycosyl hydrolase family 5 cellulase gene was overexpressed in Escherichia coli. Substrate specificity analysis revealed that this recombinant glycosyl hydrolase family 5 cellulase functions as an endo-β-1,4-glucanase. The recombinant KG35 endo-β-1,4-glucanase showed optimal activity within the range of 30-50°C at a pH of 6-7. The thermostability was retained and the pH was stable in the range of 30-50°C at a pH of 5-7.

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Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 48 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 19%
Student > Bachelor 9 19%
Researcher 4 8%
Unspecified 3 6%
Student > Master 3 6%
Other 5 10%
Unknown 15 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 21%
Agricultural and Biological Sciences 7 15%
Unspecified 3 6%
Chemistry 2 4%
Immunology and Microbiology 2 4%
Other 3 6%
Unknown 21 44%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2018.
All research outputs
#7,792,484
of 12,923,846 outputs
Outputs from Brazilian Journal of Microbiology
#178
of 434 outputs
Outputs of similar age
#139,433
of 261,456 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#3
of 12 outputs
Altmetric has tracked 12,923,846 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 434 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 261,456 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.

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