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Cationic and PEGylated Amphiphilic Cyclodextrins: Co-Formulation Opportunities for Neuronal Sirna Delivery

Overview of attention for article published in PLOS ONE, June 2013
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Cationic and PEGylated Amphiphilic Cyclodextrins: Co-Formulation Opportunities for Neuronal Sirna Delivery
Published in
PLOS ONE, June 2013
DOI 10.1371/journal.pone.0066413
Pubmed ID

Aoife M. O’Mahony, Julien Ogier, Raphael Darcy, John F. Cryan, Caitriona M. O’Driscoll


Optimising non-viral vectors for neuronal siRNA delivery presents a significant challenge. Here, we investigate a co-formulation, consisting of two amphiphilic cyclodextrins (CDs), one cationic and the other PEGylated, which were blended together for siRNA delivery to a neuronal cell culture model. Co-formulated CD-siRNA complexes were characterised in terms of size, charge and morphology. Stability in salt and serum was also examined. Uptake was determined by flow cytometry and toxicity was measured by MTT assay. Knockdown of a luciferase reporter gene was used as a measure of gene silencing efficiency. Incorporation of a PEGylated CD in the formulation had significant effects on the physical and biological properties of CD.siRNA complexes. Co-formulated complexes exhibited a lower surface charge and greater stability in a high salt environment. However, the inclusion of the PEGylated CD also dramatically reduced gene silencing efficiency due to its effects on neuronal uptake. The co-formulation strategy for cationic and PEGylated CDs improved the stability of the CD.siRNA delivery systems, although knockdown efficiency was impaired. Future work will focus on the addition of targeting ligands to the co-formulated complexes to restore transfection capabilities.

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Ireland 1 3%
Unknown 36 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 41%
Researcher 5 14%
Student > Bachelor 3 8%
Student > Master 3 8%
Student > Doctoral Student 2 5%
Other 5 14%
Unknown 4 11%
Readers by discipline Count As %
Chemistry 7 19%
Pharmacology, Toxicology and Pharmaceutical Science 5 14%
Medicine and Dentistry 5 14%
Agricultural and Biological Sciences 5 14%
Nursing and Health Professions 2 5%
Other 8 22%
Unknown 5 14%