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An att site-based recombination reporter system for genome engineering and synthetic DNA assembly

Overview of attention for article published in BMC Biotechnology, July 2017
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)

Mentioned by

twitter
9 tweeters

Readers on

mendeley
26 Mendeley
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Title
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
Published in
BMC Biotechnology, July 2017
DOI 10.1186/s12896-017-0382-1
Pubmed ID
Authors

Michael J. Bland, Magaly Ducos-Galand, Marie-Eve Val, Didier Mazel

Abstract

Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA sequences, making them valuable tools in these contexts. We have developed site-specific recombination tools that allow the direct selection of recombination events by embedding the attB site from each system within the β-lactamase resistance coding sequence (bla). The HK and ΦC31 tools were developed by placing the attB sites from each system into the signal peptide cleavage site coding sequence of bla. All possible open reading frames (ORFs) were inserted and tested for recombination efficiency and bla activity. Efficient recombination was observed for all tested ORFs (3 for HK, 6 for ΦC31) as shown through a cointegrate formation assay. The bla gene with the embedded attB site was functional for eight of the nine constructs tested. The HK/ΦC31 att-bla system offers a simple way to directly select recombination events, thus enhancing the use of site-specific recombination systems for carrying out precise, large-scale DNA manipulation, and adding useful tools to the genetics toolbox. We further show the power and flexibility of bla to be used as a reporter for recombination.

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 35%
Student > Ph. D. Student 6 23%
Student > Master 4 15%
Student > Doctoral Student 2 8%
Other 1 4%
Other 2 8%
Unknown 2 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 42%
Biochemistry, Genetics and Molecular Biology 8 31%
Immunology and Microbiology 2 8%
Medicine and Dentistry 2 8%
Psychology 1 4%
Other 0 0%
Unknown 2 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2018.
All research outputs
#4,666,281
of 15,839,539 outputs
Outputs from BMC Biotechnology
#299
of 821 outputs
Outputs of similar age
#90,782
of 267,651 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 15,839,539 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 821 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,651 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them