Chapter title |
Anther Culture in Eggplant ( Solanum melongena L.)
|
---|---|
Chapter number | 25 |
Book title |
In Vitro Embryogenesis in Higher Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3061-6_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3060-9, 978-1-4939-3061-6
|
Authors |
Giuseppe Leonardo Rotino |
Abstract |
The technique of in vitro anther culture is the most favorite to incite the production of plants from microspore through direct embryogenesis or regeneration from callus. Anther culture has been employed since 1980s in eggplant to obtain double-haploid plants from microspore derived embryos. From that time it has been refined and widely applied both at commercial level for a fast generation double-haploid parental lines of F1 hybrids, as well as for experimental studies as the complete homozygosis of the microspore-derived plants make more simply the genetic analysis. In this chapter, a step-by-step procedure is reported, taking into consideration all the aspects of the technique, including the growth condition of the anther donor plant, the in vitro regeneration of the androgenetic plantlets, their ploidy analysis, and the colchicine treatment to double the chromosome number of the haploids. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Greece | 1 | 7% |
Unknown | 13 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 36% |
Student > Ph. D. Student | 3 | 21% |
Student > Master | 1 | 7% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 64% |
Business, Management and Accounting | 1 | 7% |
Unknown | 4 | 29% |