| Title |
Total Synthesis of a Functional Designer Eukaryotic Chromosome
|
|---|---|
| Published in |
Science, March 2014
|
| DOI | 10.1126/science.1249252 |
| Pubmed ID | |
| Authors |
Narayana Annaluru, Héloïse Muller, Leslie A. Mitchell, Sivaprakash Ramalingam, Giovanni Stracquadanio, Sarah M. Richardson, Jessica S. Dymond, Zheng Kuang, Lisa Z. Scheifele, Eric M. Cooper, Yizhi Cai, Karen Zeller, Neta Agmon, Jeffrey S. Han, Michalis Hadjithomas, Jennifer Tullman, Katrina Caravelli, Kimberly Cirelli, Zheyuan Guo, Viktoriya London, Apurva Yeluru, Sindurathy Murugan, Karthikeyan Kandavelou, Nicolas Agier, Gilles Fischer, Kun Yang, J. Andrew Martin, Murat Bilgel, Pavlo Bohutskyi, Kristin M. Boulier, Brian J. Capaldo, Joy Chang, Kristie Charoen, Woo Jin Choi, Peter Deng, James E. DiCarlo, Judy Doong, Jessilyn Dunn, Jason I. Feinberg, Christopher Fernandez, Charlotte E. Floria, David Gladowski, Pasha Hadidi, Isabel Ishizuka, Javaneh Jabbari, Calvin Y. L. Lau, Pablo A. Lee, Sean Li, Denise Lin, Matthias E. Linder, Jonathan Ling, Jaime Liu, Jonathan Liu, Mariya London, Henry Ma, Jessica Mao, Jessica E. McDade, Alexandra McMillan, Aaron M. Moore, Won Chan Oh, Yu Ouyang, Ruchi Patel, Marina Paul, Laura C. Paulsen, Judy Qiu, Alex Rhee, Matthew G. Rubashkin, Ina Y. Soh, Nathaniel E. Sotuyo, Venkatesh Srinivas, Allison Suarez, Andy Wong, Remus Wong, Wei Rose Xie, Yijie Xu, Allen T. Yu, Romain Koszul, Joel S. Bader, Jef D. Boeke, Srinivasan Chandrasegaran |
| Abstract |
Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology. |
X Demographics
The data shown below were collected from the profiles of 293 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 51 | 17% |
| Spain | 27 | 9% |
| United Kingdom | 26 | 9% |
| Japan | 15 | 5% |
| France | 9 | 3% |
| Germany | 8 | 3% |
| Australia | 6 | 2% |
| Brazil | 4 | 1% |
| Netherlands | 3 | 1% |
| Other | 27 | 9% |
| Unknown | 117 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 206 | 70% |
| Scientists | 71 | 24% |
| Science communicators (journalists, bloggers, editors) | 10 | 3% |
| Practitioners (doctors, other healthcare professionals) | 6 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 972 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 22 | 2% |
| United Kingdom | 9 | <1% |
| Canada | 7 | <1% |
| France | 5 | <1% |
| Brazil | 5 | <1% |
| Germany | 5 | <1% |
| Spain | 4 | <1% |
| Japan | 3 | <1% |
| Netherlands | 3 | <1% |
| Other | 23 | 2% |
| Unknown | 886 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 230 | 24% |
| Researcher | 202 | 21% |
| Student > Bachelor | 142 | 15% |
| Student > Master | 107 | 11% |
| Professor > Associate Professor | 46 | 5% |
| Other | 155 | 16% |
| Unknown | 90 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 442 | 45% |
| Biochemistry, Genetics and Molecular Biology | 244 | 25% |
| Chemistry | 34 | 3% |
| Medicine and Dentistry | 30 | 3% |
| Engineering | 23 | 2% |
| Other | 84 | 9% |
| Unknown | 115 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 969. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2023.
All research outputs
#17,228
of 25,706,302 outputs
Outputs from Science
#850
of 83,251 outputs
Outputs of similar age
#77
of 238,784 outputs
Outputs of similar age from Science
#4
of 845 outputs
Altmetric has tracked 25,706,302 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,251 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.9. This one has done particularly well, scoring higher than 98% of its peers.
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