Title |
DAFS: a data-adaptive flag method for RNA-sequencing data to differentiate genes with low and high expression
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Published in |
BMC Bioinformatics, March 2014
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DOI | 10.1186/1471-2105-15-92 |
Pubmed ID | |
Authors |
Nysia I George, Ching-Wei Chang |
Abstract |
Next-generation sequencing (NGS) has advanced the application of high-throughput sequencing technologies in genetic and genomic variation analysis. Due to the large dynamic range of expression levels, RNA-seq is more prone to detect transcripts with low expression. It is clear that genes with no mapped reads are not expressed; however, there is ongoing debate about the level of abundance that constitutes biologically meaningful expression. To date, there is no consensus on the definition of low expression. Since random variation is high in regions with low expression and distributions of transcript expression are affected by numerous experimental factors, methods to differentiate low and high expressed data in a sample are critical to interpreting classes of abundance levels in RNA-seq data. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Norway | 1 | 11% |
Sweden | 1 | 11% |
Canada | 1 | 11% |
Germany | 1 | 11% |
Spain | 1 | 11% |
France | 1 | 11% |
United States | 1 | 11% |
Unknown | 2 | 22% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 44% |
Scientists | 4 | 44% |
Science communicators (journalists, bloggers, editors) | 1 | 11% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 5% |
Germany | 2 | 2% |
Netherlands | 1 | 1% |
Norway | 1 | 1% |
Sweden | 1 | 1% |
Italy | 1 | 1% |
United Kingdom | 1 | 1% |
Poland | 1 | 1% |
Unknown | 73 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 26 | 31% |
Researcher | 22 | 26% |
Student > Master | 15 | 18% |
Professor > Associate Professor | 6 | 7% |
Student > Bachelor | 5 | 6% |
Other | 9 | 11% |
Unknown | 2 | 2% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 45 | 53% |
Neuroscience | 10 | 12% |
Computer Science | 8 | 9% |
Biochemistry, Genetics and Molecular Biology | 6 | 7% |
Medicine and Dentistry | 3 | 4% |
Other | 9 | 11% |
Unknown | 4 | 5% |