Chapter title |
Generation of a Stress-Inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-Responsive Gene Deregulation Mutants
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Chapter number | 6 |
Book title |
Plant Stress Tolerance
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7136-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7134-3, 978-1-4939-7136-7
|
Authors |
Si-in Yu, Byeong-ha Lee |
Abstract |
In order to understand plant stress tolerance and its application, it is important to identify the signaling components involved in the stress-regulated gene expression. One initial step for this is generation of a stress-inducible luminescent Arabidopsis and its use in genetic mutant screening. Here, we describe how to generate a transgenic Arabidopsis line harboring a single copy of the STABILIZED1 (STA1) promoter-driven luciferase transgene (STA1p-LUC) as an example. STA1 is a pre-mRNA splicing factor Prp6p homolog and is induced by cold and heat stresses. In addition, generation of the STA1p-LUC mutant pool and a luminescence imaging-based screening for STA1p-LUC deregulated mutants are described. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 3% |
Unknown | 32 | 97% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 1 | 3% |
Unknown | 32 | 97% |