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Plant Stress Tolerance

Overview of attention for book
Cover of 'Plant Stress Tolerance'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Epigenetics and RNA Processing: Connections to Drought, Salt, and ABA?
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    Chapter 2 The Fundamental Role of Reactive Oxygen Species in Plant Stress Response
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    Chapter 3 The Role of Long Noncoding RNAs in Plant Stress Tolerance
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    Chapter 4 Toward a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture
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    Chapter 5 Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation
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    Chapter 6 Generation of a Stress-Inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-Responsive Gene Deregulation Mutants
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    Chapter 7 Detection of Differential DNA Methylation Under Stress Conditions Using Bisulfite Sequence Analysis
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    Chapter 8 ChIP-Seq Analysis for Identifying Genome-Wide Histone Modifications Associated with Stress-Responsive Genes in Plants
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    Chapter 9 Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants
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    Chapter 10 Global Proteomic Profiling and Identification of Stress-Responsive Proteins Using Two-Dimensional Gel Electrophoresis
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    Chapter 11 Phosphoproteomics Analysis for Probing Plant Stress Tolerance
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    Chapter 12 Probing Posttranslational Redox Modifications
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    Chapter 13 Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants
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    Chapter 14 Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays for SO, APR, SiR, and In-Gel SiR Activity
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    Chapter 15 Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants
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    Chapter 16 Determining Glutathione Levels in Plants
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    Chapter 17 Porous Graphitic Carbon Liquid Chromatography–Mass Spectrometry Analysis of Drought Stress-Responsive Raffinose Family Oligosaccharides in Plant Tissues
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    Chapter 18 Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses
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    Chapter 19 Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC)
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    Chapter 20 Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis
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    Chapter 21 Rapid Quantification of Abscisic Acid by GC-MS/MS for Studies of Abiotic Stress Response
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    Chapter 22 Silencing of Stress-Regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach
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    Chapter 23 Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes
Attention for Chapter 17: Porous Graphitic Carbon Liquid Chromatography–Mass Spectrometry Analysis of Drought Stress-Responsive Raffinose Family Oligosaccharides in Plant Tissues
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Chapter title
Porous Graphitic Carbon Liquid Chromatography–Mass Spectrometry Analysis of Drought Stress-Responsive Raffinose Family Oligosaccharides in Plant Tissues
Chapter number 17
Book title
Plant Stress Tolerance
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7136-7_17
Pubmed ID
Book ISBNs
978-1-4939-7134-3, 978-1-4939-7136-7
Authors

Tiago F. Jorge, Maria H. Florêncio, Carla António, Jorge, Tiago F., Florêncio, Maria H., António, Carla

Abstract

Drought is a major limiting factor in agriculture and responsible for dramatic crop yield losses worldwide. The adjustment of the metabolic status via accumulation of drought stress-responsive osmolytes is one of the many strategies that some plants have developed to cope with water deficit conditions. Osmolytes are highly polar compounds, analysis of whcih is difficult with typical reversed-phase chromatography. Porous graphitic carbon (PGC) has shown to be a suitable alternative to reversed-phase stationary phases for the analysis of highly polar compounds typically found in the plant metabolome. In this chapter, we describe the development and validation of a PGC-based liquid chromatography tandem mass spectrometry (LC-MS(n)) method suitable for the target analysis of water-soluble carbohydrates, such as raffinose family oligosaccharides (RFOs). We present detailed information regarding PGC column equilibration, LC-MS(n) system operation, data analysis, and important notes to be considered during the steps of method development and validation.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 29%
Researcher 2 29%
Student > Bachelor 1 14%
Student > Master 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Chemistry 1 14%
Unknown 2 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2017.
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#20,436,330
of 22,990,068 outputs
Outputs from Methods in molecular biology
#9,932
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Outputs of similar age
#275,925
of 316,114 outputs
Outputs of similar age from Methods in molecular biology
#190
of 249 outputs
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We're also able to compare this research output to 249 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.