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Plant Stress Tolerance

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Cover of 'Plant Stress Tolerance'

Table of Contents

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    Book Overview
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    Chapter 1 Epigenetics and RNA Processing: Connections to Drought, Salt, and ABA?
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    Chapter 2 The Fundamental Role of Reactive Oxygen Species in Plant Stress Response
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    Chapter 3 The Role of Long Noncoding RNAs in Plant Stress Tolerance
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    Chapter 4 Toward a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture
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    Chapter 5 Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation
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    Chapter 6 Generation of a Stress-Inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-Responsive Gene Deregulation Mutants
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    Chapter 7 Detection of Differential DNA Methylation Under Stress Conditions Using Bisulfite Sequence Analysis
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    Chapter 8 ChIP-Seq Analysis for Identifying Genome-Wide Histone Modifications Associated with Stress-Responsive Genes in Plants
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    Chapter 9 Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants
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    Chapter 10 Global Proteomic Profiling and Identification of Stress-Responsive Proteins Using Two-Dimensional Gel Electrophoresis
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    Chapter 11 Phosphoproteomics Analysis for Probing Plant Stress Tolerance
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    Chapter 12 Probing Posttranslational Redox Modifications
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    Chapter 13 Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants
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    Chapter 14 Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays for SO, APR, SiR, and In-Gel SiR Activity
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    Chapter 15 Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants
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    Chapter 16 Determining Glutathione Levels in Plants
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    Chapter 17 Porous Graphitic Carbon Liquid Chromatography–Mass Spectrometry Analysis of Drought Stress-Responsive Raffinose Family Oligosaccharides in Plant Tissues
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    Chapter 18 Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses
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    Chapter 19 Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC)
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    Chapter 20 Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis
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    Chapter 21 Rapid Quantification of Abscisic Acid by GC-MS/MS for Studies of Abiotic Stress Response
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    Chapter 22 Silencing of Stress-Regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach
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    Chapter 23 Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes
Attention for Chapter 21: Rapid Quantification of Abscisic Acid by GC-MS/MS for Studies of Abiotic Stress Response
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Chapter title
Rapid Quantification of Abscisic Acid by GC-MS/MS for Studies of Abiotic Stress Response
Chapter number 21
Book title
Plant Stress Tolerance
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7136-7_21
Pubmed ID
Book ISBNs
978-1-4939-7134-3, 978-1-4939-7136-7
Authors

Paul E. Verslues

Abstract

Drought and low water potential induce large increases in Abscisic Acid (ABA ) content of plant tissue. This increased ABA content is essential to regulate downstream stress resistance responses; however, the mechanisms regulating ABA accumulation are incompletely known. Thus, the ability to accurately quantify ABA at high throughput and low cost is important for plant stress research. We have combined and modified several previously published protocols to establish a rapid ABA analysis protocol using gas chromatography-tandem mass spectrometry (GC-MS/MS). Derivatization of ABA is performed with (trimethylsilyl)-diazomethane rather than the harder to prepare diazomethane. Sensitivity of the analysis is sufficient that small samples of low water potential treated Arabidopsis thaliana seedlings can be routinely analyzed in reverse genetic studies of putative stress regulators as well as studies of natural variation in ABA accumulation.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 19%
Student > Ph. D. Student 6 19%
Student > Bachelor 3 10%
Student > Master 3 10%
Student > Doctoral Student 2 6%
Other 1 3%
Unknown 10 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 29%
Biochemistry, Genetics and Molecular Biology 8 26%
Environmental Science 2 6%
Chemical Engineering 1 3%
Psychology 1 3%
Other 1 3%
Unknown 9 29%