Chapter title |
Rapid Quantification of Abscisic Acid by GC-MS/MS for Studies of Abiotic Stress Response
|
---|---|
Chapter number | 21 |
Book title |
Plant Stress Tolerance
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7136-7_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7134-3, 978-1-4939-7136-7
|
Authors |
Paul E. Verslues |
Abstract |
Drought and low water potential induce large increases in Abscisic Acid (ABA ) content of plant tissue. This increased ABA content is essential to regulate downstream stress resistance responses; however, the mechanisms regulating ABA accumulation are incompletely known. Thus, the ability to accurately quantify ABA at high throughput and low cost is important for plant stress research. We have combined and modified several previously published protocols to establish a rapid ABA analysis protocol using gas chromatography-tandem mass spectrometry (GC-MS/MS). Derivatization of ABA is performed with (trimethylsilyl)-diazomethane rather than the harder to prepare diazomethane. Sensitivity of the analysis is sufficient that small samples of low water potential treated Arabidopsis thaliana seedlings can be routinely analyzed in reverse genetic studies of putative stress regulators as well as studies of natural variation in ABA accumulation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 31 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 19% |
Student > Ph. D. Student | 6 | 19% |
Student > Bachelor | 3 | 10% |
Student > Master | 3 | 10% |
Student > Doctoral Student | 2 | 6% |
Other | 1 | 3% |
Unknown | 10 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 29% |
Biochemistry, Genetics and Molecular Biology | 8 | 26% |
Environmental Science | 2 | 6% |
Chemical Engineering | 1 | 3% |
Psychology | 1 | 3% |
Other | 1 | 3% |
Unknown | 9 | 29% |