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In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai

Overview of attention for article published in Journal of Venomous Animals and Toxins including Tropical Diseases, July 2017
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2 tweeters
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1 Facebook page

Citations

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2 Dimensions

Readers on

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11 Mendeley
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1 CiteULike
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Title
In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai
Published in
Journal of Venomous Animals and Toxins including Tropical Diseases, July 2017
DOI 10.1186/s40409-017-0125-8
Pubmed ID
Authors

Seong Kyeong Bae, Hyunkyoung Lee, Yunwi Heo, Min Jung Pyo, Indu Choudhary, Chang Hoon Han, Won Duk Yoon, Changkeun Kang, Euikyung Kim

Abstract

Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a serine protease component of Nemopilema nomurai jellyfish venom (NnV) and unveil its characteristics. To determine the relationship between fibrinolytic activity of NnV and the serine protease, fibrin zymography was performed using metalloprotease and serine protease inhibitors. The biochemical characterization of serine proteases of NnV were determined by the amidolytic assay. Fractions with fibrinolytic activity were obtained by DEAE cation exchange column. NnV displayed fibrinolytic activities with molecular masses of approximately 70, 35, 30, and 28 kDa. The fibrinolytic activity of NnV was completely obliterated by phenylmethylsulfonyl fluoride, a prototype serine protease inhibitor. Based on amidolytic assays using chromogenic substrates specific for various kinds of serine proteases, NnV predominantly manifested a chymotrypsin-like feature. Its activity was completely eliminated at low pH (< 6) and high temperatures (> 37 °C). Some metal ions (Co(2+), Cu(2+), Zn(2+) and Ni(2+)) strongly suppressed its fibrinolytic activity, while others (Ca(2+) and Mg(2+)) failed to do so. Isolation of a serine protease with fibrionolytic activity from NnV revealed that only p3 showed the fibrinolytic activity, which was completely inhibited by PMSF. The present study showed that N. nomurai jellyfish venom has a chymotrypsin-like serine protease with fibrinolytic activity. Such information might be useful for developing clinical management of jellyfish envenomation and pharmacological agents with therapeutic potential for thrombotic diseases in the future.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 18%
Student > Master 2 18%
Student > Ph. D. Student 2 18%
Professor 1 9%
Student > Doctoral Student 1 9%
Other 0 0%
Unknown 3 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 27%
Biochemistry, Genetics and Molecular Biology 2 18%
Business, Management and Accounting 1 9%
Arts and Humanities 1 9%
Earth and Planetary Sciences 1 9%
Other 0 0%
Unknown 3 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2017.
All research outputs
#6,556,713
of 11,511,689 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#120
of 300 outputs
Outputs of similar age
#126,326
of 262,616 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#4
of 10 outputs
Altmetric has tracked 11,511,689 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 300 research outputs from this source. They receive a mean Attention Score of 3.2. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,616 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.