Chapter title |
Preparation of a Conditional RNA Switch
|
---|---|
Chapter number | 20 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7138-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7137-4, 978-1-4939-7138-1
|
Authors |
Zakrevsky, Paul, Parlea, Lorena, Viard, Mathias, Bindewald, Eckart, Afonin, Kirill A., Shapiro, Bruce A., Paul Zakrevsky, Lorena Parlea, Mathias Viard, Eckart Bindewald, Kirill A. Afonin, Bruce A. Shapiro |
Abstract |
RNA has gained great interest for use in biomedical and therapeutic applications. This is due in part to RNA's ability to perform multiple functions, including the regulation of endogenously expressed genes. However, the ability of RNA based drugs to distinguish target diseased cells from healthy tissue remains challenging. Here we present methods for the production of a recently developed conditional RNA switch that releases a Dicer substrate RNA in response to interaction with a specific RNA biomarker. |
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