Chapter title |
Synthesis of PLGA–Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG–Lipid Nanoparticles for siRNA Delivery
|
---|---|
Chapter number | 15 |
Book title |
RNA Nanostructures
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7138-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7137-4, 978-1-4939-7138-1
|
Authors |
Lei Wang, Benjamin Griffel, Xiaoyang Xu |
Abstract |
The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG-cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo. |
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