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Rhinoviruses

Overview of attention for book
Cover of 'Rhinoviruses'

Table of Contents

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    Book Overview
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    Chapter 1 Classification and Evolution of Human Rhinoviruses
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    Chapter 2 Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
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    Chapter 3 Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples
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    Chapter 4 Molecular genotyping of human rhinovirus by using PCR and sanger sequencing.
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    Chapter 5 Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions
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    Chapter 6 Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface
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    Chapter 7 Infectivity Assays of Human Rhinovirus-A and -B Serotypes
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    Chapter 8 Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating
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    Chapter 9 Capillary Electrophoresis, Gas-Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy: Effective Tools for Quality Assessment and Basic Rhinovirus Research
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    Chapter 10 Proteases of Human Rhinovirus: Role in Infection
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    Chapter 11 A Protocol to Express and Isolate HRV16 3C Protease for Use in Protease Assays
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    Chapter 12 Reverse Genetics System for Studying Human Rhinovirus Infections
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    Chapter 13 Reverse genetic engineering of the human rhinovirus serotype 16 genome to introduce an antibody-detectable tag.
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    Chapter 14 Mouse Models of Rhinovirus Infection and Airways Disease
Attention for Chapter 4: Molecular genotyping of human rhinovirus by using PCR and sanger sequencing.
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Chapter title
Molecular genotyping of human rhinovirus by using PCR and sanger sequencing.
Chapter number 4
Book title
Rhinoviruses
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1571-2_4
Pubmed ID
Book ISBNs
978-1-4939-1570-5, 978-1-4939-1571-2
Authors

Wei Wang, Jing He, Yi Liu, Lei Xu, Wencai Guan, Yunwen Hu

Abstract

Human rhinovirus (HRV) is the virus most often associated with acute upper respiratory tract infections. Advances in molecular detection have shown that HRV is also the major viral cause of asthma exacerbations. Genotypic assignment and identification of HRV types are of significant value in the investigation of type-associated differences in disease outcomes, transmission, and epidemiology. Here, we describe a genotyping process involving two separate RT-PCR assays, targeted to VP4/VP2 and 5' UTR regions of HRV genome, respectively. Together with the reference sequences of each HRV species, the generated sequences are used to construct phylogenetic tree for genotyping.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 4 20%
Student > Doctoral Student 3 15%
Student > Bachelor 3 15%
Student > Ph. D. Student 3 15%
Student > Master 2 10%
Other 5 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 30%
Immunology and Microbiology 4 20%
Engineering 3 15%
Medicine and Dentistry 3 15%
Biochemistry, Genetics and Molecular Biology 2 10%
Other 1 5%
Unknown 1 5%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2014.
All research outputs
#20,237,640
of 22,764,165 outputs
Outputs from Methods in molecular biology
#9,866
of 13,089 outputs
Outputs of similar age
#295,617
of 352,895 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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