Title |
A salt-bridge structure in solution revealed by 2D-IR spectroscopy
|
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Published in |
Journal of the Chemical Society, Faraday Transactions, January 2014
|
DOI | 10.1039/c4cp00233d |
Pubmed ID | |
Authors |
Adriana Huerta-Viga, Sérgio R. Domingos, Saeed Amirjalayer, Sander Woutersen |
Abstract |
Salt bridges are important interactions for the stability of protein conformations, but up to now it has been difficult to determine salt-bridge geometries in solution. Here we characterize the spatial structure of a salt bridge between guanidinium (Gdm(+)) and acetate (Ac(-)) using two-dimensional vibrational (2D-IR) spectroscopy. We find that as a result of salt bridge formation there is a significant change in the infrared response of Gdm(+) and Ac(-), and cross peaks between them appear in the 2D-IR spectrum. From the 2D-IR spectrum we determine the relative orientation of the transition-dipole moments of the vibrational modes of Gdm(+) and Ac(-), as well as the coupling between them. |
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