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Gene expression analysis of the microvascular compartment in multiple sclerosis using laser microdissected blood vessels

Overview of attention for article published in Acta Neuropathologica, December 2009
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  • Above-average Attention Score compared to outputs of the same age (63rd percentile)

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2 patents


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34 Mendeley
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Gene expression analysis of the microvascular compartment in multiple sclerosis using laser microdissected blood vessels
Published in
Acta Neuropathologica, December 2009
DOI 10.1007/s00401-009-0618-9
Pubmed ID

Paula Cunnea, Jill McMahon, Enda O’Connell, Kaveh Mashayekhi, Una Fitzgerald, Stephen McQuaid


The blood brain barrier (BBB) is formed by capillary endothelial cells with inter-endothelial cell tight junctions and other cells such as pericytes and astrocytes present. Previous studies have shown a role for tight junction abnormalities in BBB leakage in multiple sclerosis (MS) brain. This marks a key stage in the development of inflammatory demyelination in MS. The aim of this study was to identify aberrantly expressed genes involved in BBB changes in MS lesions. A focused endothelial cell biology microarray, capable of detecting changes in expression of 113 endothelial cell-specific genes, was employed to analyse endothelial cell mRNA extracted from post-mortem control white matter, MS normal appearing white matter (NAWM), chronic active or inactive lesions by laser capture microdissection. Microarray analysis found 52 genes out of 113 analysed, predominantly in the activation functional group, to be differentially expressed in lesions compared to control or NAWM (p < 0.01). The majority of the differentially expressed genes were validated by quantitative real time PCR. In addition, the protein expression profiles of ICAM2, MMP2, and VEGFR1 were examined by immunofluorescent staining of selected tissue blocks. ICAM-2 was expressed at a higher level in chronic inactive lesions than control or NAWM, corresponding with the increased mRNA measured by microarray and real time PCR. The data shown, presenting a number of differentially expressed genes in the microvascular compartment of MS lesions, may shed light on the molecular mechanisms that are involved in the breakdown of the BBB. This moves us a step closer to the identification of potential therapeutic targets for repair of the compromised BBB.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 3%
United Kingdom 1 3%
Unknown 32 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 26%
Student > Ph. D. Student 6 18%
Student > Master 5 15%
Student > Bachelor 4 12%
Other 3 9%
Other 7 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 26%
Biochemistry, Genetics and Molecular Biology 7 21%
Medicine and Dentistry 6 18%
Neuroscience 6 18%
Unspecified 3 9%
Other 3 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 August 2017.
All research outputs
of 11,667,520 outputs
Outputs from Acta Neuropathologica
of 1,470 outputs
Outputs of similar age
of 266,017 outputs
Outputs of similar age from Acta Neuropathologica
of 35 outputs
Altmetric has tracked 11,667,520 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,470 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.6. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,017 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.