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Cross-Linking of β-Lactoglobulin Enhances Allergic Sensitization Through Changes in Cellular Uptake and Processing

Overview of attention for article published in Toxicological Sciences, April 2014
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (56th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

Mentioned by

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3 tweeters

Citations

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40 Dimensions

Readers on

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60 Mendeley
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Title
Cross-Linking of β-Lactoglobulin Enhances Allergic Sensitization Through Changes in Cellular Uptake and Processing
Published in
Toxicological Sciences, April 2014
DOI 10.1093/toxsci/kfu062
Pubmed ID
Authors

Marija Stojadinovic, Raymond Pieters, Joost Smit, Tanja Cirkovic Velickovic

Abstract

Cross-linking of proteins has been exploited by the food industry to change food texture and functionality but the effects of these manipulations on food allergenicity still remain unclear. To model the safety assessment of these food biopolymers, we created cross-linked bovine β-lactoglobulin (CL-BLG) by laccase treatment. The purpose of the present study was to compare the immunogenicity and allergenicity of CL-BLG with native BLG in a mouse model of food allergy. First, BALB/c mice were intragastrically sensitized and orally challenged with BLG or CL-BLG and BLG-specific serum antibodies and splenic leukocyte cytokine production and cell proliferation were measured. Hereafter, epithelial protein uptake was monitored in vitro and in vivo and the effects of BLG cross-linking on interactions with dendritic cells were analyzed in vitro. Sensitization of mice with CL-BLG resulted in higher levels of IgE, IgG1, and IgG2a. In contrast, a subsequent oral challenge with CL-BLG resulted in lower mast cell degranulation. Cross-linking of BLG reduced its epithelial uptake but promoted sampling through Peyer's patches. Differences in endocytosis by dendritic cells (DCs) and in vitro endolysosomal processing were observed between BLG and CL-BLG. CL-BLG primed DCs induced higher Th2 response in vitro. Cross-linking of BLG increased its sensitizing capacity, implying that the assessment of highly polymerized food proteins is of clinical importance in food allergy. Moreover, manufacturers of foods or therapeutic proteins should pay considerate attention to the health risk of protein aggregation.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 2%
France 1 2%
Unknown 58 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 22%
Student > Ph. D. Student 12 20%
Student > Master 12 20%
Student > Bachelor 5 8%
Professor > Associate Professor 2 3%
Other 5 8%
Unknown 11 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 23%
Immunology and Microbiology 8 13%
Biochemistry, Genetics and Molecular Biology 7 12%
Medicine and Dentistry 5 8%
Chemistry 4 7%
Other 7 12%
Unknown 15 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2015.
All research outputs
#6,765,944
of 12,029,804 outputs
Outputs from Toxicological Sciences
#2,897
of 3,638 outputs
Outputs of similar age
#80,972
of 192,504 outputs
Outputs of similar age from Toxicological Sciences
#13
of 36 outputs
Altmetric has tracked 12,029,804 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,638 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 192,504 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.