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Mendeley readers
Chapter title |
Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine
|
---|---|
Chapter number | 13 |
Book title |
mRNA Processing
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7204-3_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7203-6, 978-1-4939-7204-3
|
Authors |
Angela Garibaldi, Francisco Carranza, Klemens J. Hertel |
Abstract |
Isolation of newly transcribed RNA is an invaluable approach that can be used to study the dynamic life of RNA in cellulo. Traditional methods of whole-cell RNA extraction limit subsequent gene expression analyses to the steady-state levels of RNA abundance, which often masks changes in RNA synthesis and processing. This chapter describes a methodology with low cytotoxicity that permits the labeling and isolation of nascent pre-mRNA in cell culture. The resulting isolate is suitable for use in a series of downstream applications aimed at studying changes in RNA synthesis, processing, or stability. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 67 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 31% |
Researcher | 8 | 12% |
Professor > Associate Professor | 4 | 6% |
Student > Master | 4 | 6% |
Student > Doctoral Student | 3 | 4% |
Other | 9 | 13% |
Unknown | 18 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 31 | 46% |
Agricultural and Biological Sciences | 7 | 10% |
Immunology and Microbiology | 3 | 4% |
Computer Science | 2 | 3% |
Engineering | 2 | 3% |
Other | 6 | 9% |
Unknown | 16 | 24% |