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Mendeley readers
| Chapter title |
Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells
|
|---|---|
| Chapter number | 12 |
| Book title |
mRNA Processing
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7204-3_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7203-6, 978-1-4939-7204-3
|
| Authors |
Kyu-Hyeon Yeom, Andrey Damianov |
| Abstract |
Eukaryotic organelles or subcellular compartments can be selectively isolated based on their physical density and their stability in the presence of nonionic detergents. This chapter describes a protocol for the preparation of cytoplasm, nucleoplasm, and chromatin, in addition to isolation of RNA and proteins from these fractions. Proteins and protein complexes stably associated with chromatin and other high molecular weight nuclear components can be extracted under non-denaturing conditions by enzymatic digestion of RNA and DNA. The chapter also includes a detailed extraction protocol from highly purified nuclei. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 50% |
| Student > Doctoral Student | 2 | 25% |
| Student > Ph. D. Student | 1 | 13% |
| Student > Bachelor | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 63% |
| Agricultural and Biological Sciences | 2 | 25% |
| Unknown | 1 | 13% |