Chapter 1 Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction

Chapter 2 Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay

Chapter 3 Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS)

Chapter 4 In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides

Chapter 5 Purification of mRNA Processing Complexes Using an RNA Affinity Approach

Chapter 6 Polyadenylation Site-Based Analysis of Transcript Expression by 3′READS+

Chapter 7 Comprehensive Identification of mRNA Polyadenylation Sites by PAPERCLIP

Chapter 8 Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells

Chapter 9 Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA

Chapter 10 Using RNA-Seq to Discover Genetic Polymorphisms That Produce Hidden Splice Variants

Chapter 11 Inducible Expression of Eukaryotic Circular RNAs from Plasmids

Chapter 12 Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells

Chapter 13 Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine

Chapter 14 Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)

Chapter 15 Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae

Chapter 16 A Cell-Based High-Throughput Method for Identifying Modulators of Alternative Splicing

Chapter 17 Genome-Wide RNAi Screens for RNA Processing Events in Drosophila melanogaster S2 Cells

Chapter 18 Assaying RNA Structure Inside Living Cells with SHAPE

Chapter 19 Erratum to: Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)