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Surfactant effects on the viability and function of human mesenchymal stem cells: in vitro and in vivo assessment

Overview of attention for article published in Stem Cell Research & Therapy, August 2017
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Title
Surfactant effects on the viability and function of human mesenchymal stem cells: in vitro and in vivo assessment
Published in
Stem Cell Research & Therapy, August 2017
DOI 10.1186/s13287-017-0634-y
Pubmed ID
Authors

Chung-Ming Chen, Hsiu-Chu Chou, Willie Lin, Chris Tseng, Chung-Ming Chen, Hsiu-Chu Chou, Willie Lin, Chris Tseng

Abstract

Surfactant therapy has become the standard of care for preterm infants with respiratory distress syndrome. Preclinical studies have reported the therapeutic benefits of mesenchymal stem cells (MSCs) in experimental bronchopulmonary dysplasia. This study investigated the effects of a surfactant on the in vitro viability and in vivo function of human MSCs. The viability, phenotype, and mitochondrial membrane potential (MMP) of MSCs were assessed through flow cytometry. The in vivo function was assessed after intratracheal injection of human MSCs (1 × 10(5) cells) diluted in 30 μl of normal saline (NS), 10 μl of a surfactant diluted in 20 μl of NS, and 10 μl of a surfactant and MSCs (1 × 10(5) cells) diluted in 20 μl of NS in newborn rats on postnatal day 5. The pups were reared in room air (RA) or an oxygen-enriched atmosphere (85% O2) from postnatal days 1 to 14; eight study groups were examined: RA + NS, RA + MSCs, RA + surfactant, RA + surfactant + MSCs, O2 + NS, O2 + MSCs, O2 + surfactant, and O2 + surfactant + MSCs. The lungs were excised for histological and cytokine analysis on postnatal day 14. Compared with the controls, surfactant-treated MSCs showed significantly reduced viability and MMP after exposure to 1:1 and 1:2 of surfactant:MSCs for 15 and 60 minutes. All human MSC samples exhibited similar percentages of CD markers, regardless of surfactant exposure. The rats reared in hyperoxia and treated with NS exhibited a significantly higher mean linear intercept (MLI) than did those reared in RA and treated with NS, MSCs, surfactant, or surfactant + MSCs. Treatment with MSCs, surfactant, or surfactant + MSCs significantly reduced the hyperoxia-induced increase in MLI. The O2 + surfactant + MSCs group exhibited a significantly higher MLI than did the O2 + MSCs group. Furthermore, treatment with MSCs and MSCs + surfactant significantly reduced the hyperoxia-induced increase in apoptotic cells. Combination therapy involving a surfactant and MSCs does not exert additive effects on lung development in hyperoxia-induced lung injury.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 16%
Student > Bachelor 4 13%
Student > Ph. D. Student 3 10%
Other 2 6%
Student > Postgraduate 2 6%
Other 6 19%
Unknown 9 29%
Readers by discipline Count As %
Medicine and Dentistry 9 29%
Biochemistry, Genetics and Molecular Biology 4 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Unspecified 1 3%
Nursing and Health Professions 1 3%
Other 3 10%
Unknown 12 39%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2017.
All research outputs
#9,253,730
of 11,564,189 outputs
Outputs from Stem Cell Research & Therapy
#705
of 939 outputs
Outputs of similar age
#194,756
of 265,536 outputs
Outputs of similar age from Stem Cell Research & Therapy
#38
of 47 outputs
Altmetric has tracked 11,564,189 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 939 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,536 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.