↓ Skip to main content

Cardiogenesis of Embryonic Stem Cells with Liquid Marble Micro‐Bioreactor

Overview of attention for article published in Advanced Healthcare Materials, May 2014
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

wikipedia
1 Wikipedia page

Citations

dimensions_citation
48 Dimensions

Readers on

mendeley
45 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Cardiogenesis of Embryonic Stem Cells with Liquid Marble Micro‐Bioreactor
Published in
Advanced Healthcare Materials, May 2014
DOI 10.1002/adhm.201400138
Pubmed ID
Authors

Sarvi F, Jain K, Arbatan T, Verma PJ, Hourigan K, Thompson MC, Shen W, Chan PP, Sarvi, Fatemeh, Jain, Kanika, Arbatan, Tina, Verma, Paul J., Hourigan, Kerry, Thompson, Mark C., Shen, Wei, Chan, Peggy P. Y., Fatemeh Sarvi, Kanika Jain, Tina Arbatan, Paul J. Verma, Kerry Hourigan, Mark C. Thompson, Wei Shen, Peggy P. Y. Chan

Abstract

A liquid marble micro-bioreactor is prepared by placing a drop of murine embryonic stem cell (ESC) (Oct4B2-ESC) suspension onto a polytetrafluoroethylene (PTFE) particle bed. The Oct4B2-ESC aggregates to form embryoid bodies (EBs) with relatively uniform size and shape in a liquid marble within 3 d. For the first time, the feasibility of differentiating ESC into cardiac lineages within liquid marbles is being investigated. Without the addition of growth factors, suspended EBs from liquid marbles express various precardiac mesoderm markers including Flk-1, Gata4, and Nkx2.5. Some of the suspended EBs exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac mesoderm differentiation. Some of the EBs are subsequently plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiac markers atrial myosin light chain 2a (MLC2a) and ventricular myosin light chain (MLC2v), and the cardiac structural marker α-actinin. More than 60% of the plated EBs exhibit spontaneous contraction and express mature cardiomyocyte marker cardiac troponin T (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. Together, these results demonstrate that the liquid-marble technique is an easily employed, cost effective, and efficient approach to generate EBs and facilitating their cardiogenesis.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 22%
Researcher 8 18%
Professor 5 11%
Student > Master 5 11%
Student > Bachelor 4 9%
Other 9 20%
Unknown 4 9%
Readers by discipline Count As %
Engineering 9 20%
Agricultural and Biological Sciences 6 13%
Veterinary Science and Veterinary Medicine 5 11%
Chemistry 5 11%
Biochemistry, Genetics and Molecular Biology 4 9%
Other 11 24%
Unknown 5 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2018.
All research outputs
#3,977,416
of 13,370,991 outputs
Outputs from Advanced Healthcare Materials
#667
of 1,331 outputs
Outputs of similar age
#54,592
of 188,384 outputs
Outputs of similar age from Advanced Healthcare Materials
#18
of 38 outputs
Altmetric has tracked 13,370,991 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,331 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.8. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 188,384 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.