You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging
|
|---|---|
| Published in |
Scientific Reports, August 2017
|
| DOI | 10.1038/s41598-017-07820-6 |
| Pubmed ID | |
| Authors |
Kazuki Harada, Motoki Ito, Xiaowen Wang, Mika Tanaka, Devina Wongso, Ayumu Konno, Hirokazu Hirai, Hajime Hirase, Takashi Tsuboi, Tetsuya Kitaguchi |
| Abstract |
cAMP is a common second messenger that is involved in various physiological processes. To expand the colour palette of available cAMP indicators, we developed a red cAMP indicator named "Pink Flamindo" (Pink Fluorescent cAMP indicator). The fluorescence intensity of Pink Flamindo increases 4.2-fold in the presence of a saturating dose of cAMP, with excitation and emission peaks at 567 nm and 590 nm, respectively. Live-cell imaging revealed that Pink Flamindo is effective for monitoring the spatio-temporal dynamics of intracellular cAMP generated by photoactivated adenylyl cyclase in response to blue light, and in dual-colour imaging studies using a green Ca(2+) indicator (G-GECO). Furthermore, we successfully monitored the elevation of cAMP levels in vivo in cerebral cortical astrocytes by two-photon imaging. We propose that Pink Flamindo will facilitate future in vivo, optogenetic studies of cell signalling and cAMP dynamics. |
X Demographics
The data shown below were collected from the profiles of 63 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 27 | 43% |
| Canada | 8 | 13% |
| Japan | 3 | 5% |
| France | 2 | 3% |
| China | 2 | 3% |
| South Africa | 1 | 2% |
| Italy | 1 | 2% |
| Austria | 1 | 2% |
| Thailand | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 15 | 24% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 44 | 70% |
| Scientists | 17 | 27% |
| Practitioners (doctors, other healthcare professionals) | 1 | 2% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 300 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 300 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 66 | 22% |
| Researcher | 45 | 15% |
| Student > Master | 28 | 9% |
| Student > Bachelor | 26 | 9% |
| Professor | 17 | 6% |
| Other | 45 | 15% |
| Unknown | 73 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 67 | 22% |
| Biochemistry, Genetics and Molecular Biology | 62 | 21% |
| Agricultural and Biological Sciences | 49 | 16% |
| Chemistry | 11 | 4% |
| Engineering | 6 | 2% |
| Other | 20 | 7% |
| Unknown | 85 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 48. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2023.
All research outputs
#879,562
of 25,635,728 outputs
Outputs from Scientific Reports
#9,340
of 142,172 outputs
Outputs of similar age
#17,868
of 328,008 outputs
Outputs of similar age from Scientific Reports
#387
of 6,015 outputs
Altmetric has tracked 25,635,728 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 142,172 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.8. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,008 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 6,015 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.