Chapter title |
Assessing Pseudomonas Virulence with a Nonmammalian Host: Drosophila melanogaster.
|
---|---|
Chapter number | 56 |
Book title |
Pseudomonas Methods and Protocols
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0473-0_56 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0472-3, 978-1-4939-0473-0
|
Authors |
Samantha Haller, Stefanie Limmer, Dominique Ferrandon, Haller, Samantha, Limmer, Stefanie, Ferrandon, Dominique |
Abstract |
Drosophila melanogaster flies represent an interesting model to study host-pathogen interactions as: (1) they are cheap and easy to raise rapidly and do not bring up ethical issues, (2) available genetic tools are highly sophisticated, for instance allowing tissue-specific alteration of gene expression, e.g., of immune genes, (3) they have a relatively complex organization, with distinct digestive tract and body cavity in which local or systemic infections, respectively, take place, (4) a medium throughput can be achieved in genetic screens, for instance looking for Pseudomonas aeruginosa mutants with altered virulence. We present here the techniques used to investigate host-pathogen relationships, namely the two major models of infections as well as the relevant parameters used to monitor the infection (survival, bacterial titer, induction of host immune response). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 6% |
Unknown | 29 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 23% |
Researcher | 5 | 16% |
Student > Bachelor | 3 | 10% |
Student > Master | 3 | 10% |
Student > Postgraduate | 2 | 6% |
Other | 5 | 16% |
Unknown | 6 | 19% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 5 | 16% |
Biochemistry, Genetics and Molecular Biology | 3 | 10% |
Medicine and Dentistry | 2 | 6% |
Nursing and Health Professions | 1 | 3% |
Other | 2 | 6% |
Unknown | 7 | 23% |