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Mimicking the Biochemical and Mechanical Extracellular Environment of the Endochondral Ossification Process to Enhance the In Vitro Mineralization Potential…

Overview of attention for article published in Tissue Engineering: Part A, December 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (78th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

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10 tweeters

Citations

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10 Dimensions

Readers on

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32 Mendeley
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Title
Mimicking the Biochemical and Mechanical Extracellular Environment of the Endochondral Ossification Process to Enhance the In Vitro Mineralization Potential of Human Mesenchymal Stem Cells
Published in
Tissue Engineering: Part A, December 2017
DOI 10.1089/ten.tea.2017.0052
Pubmed ID
Authors

Fiona E. Freeman, Jessica Schiavi, Meadhbh A. Brennan, Peter Owens, Pierre Layrolle, Laoise M. McNamara

Abstract

Chondrogenesis and mechanical stimulation of the cartilage template, are essential for bone formation via the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis. In this study we test the hypothesis that mimicking both the biochemical and mechanical extracellular environment arising during endochondral ossification can enhance the in vitro mineralization potential of human Mesenchymal Stem Cells (hMSCs). hMSC cellular aggregates were cultured for 21 days under the following culture conditions; (1) Growth Medium-Hydrostatic Pressure (HP), (2) Chondrogenic Priming -HP, (3) Growth Medium +HP, (4) Chondrogenic Priming +HP. Each group was then further cultured for another 21 days in the presence of osteogenic growth factors without HP. Biochemical (DNA, sGAG, hydroxyproline, ALP, and calcium), histological (Alcian Blue, Alizarin Red), and immunohistological (Col I, II, X, BSP-2) analyses were conducted to investigate chondrogenic and osteogenic differentiation at various time points (14, 21, 35 and 42 days). Our results showed the application of HP induced chondrogenesis similar to that of chondrogenic priming but interestingly there was a reduction in hypertrophy markers (Collagen Type X) by applying HP alone versus chondrogenic priming alone. Moreover, the results showed that both chondrogenic priming and HP in tandem during the priming period, followed by culture in osteogenic medium accelerated the osteogenic potential of hMSCs.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 28%
Researcher 6 19%
Student > Bachelor 3 9%
Student > Master 3 9%
Student > Doctoral Student 1 3%
Other 1 3%
Unknown 9 28%
Readers by discipline Count As %
Engineering 7 22%
Medicine and Dentistry 6 19%
Agricultural and Biological Sciences 3 9%
Biochemistry, Genetics and Molecular Biology 1 3%
Materials Science 1 3%
Other 1 3%
Unknown 13 41%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2018.
All research outputs
#1,823,648
of 12,413,055 outputs
Outputs from Tissue Engineering: Part A
#113
of 1,296 outputs
Outputs of similar age
#56,998
of 268,472 outputs
Outputs of similar age from Tissue Engineering: Part A
#7
of 27 outputs
Altmetric has tracked 12,413,055 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,296 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,472 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.