Title |
The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress
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Published in |
Molecular Brain, August 2017
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DOI | 10.1186/s13041-017-0318-7 |
Pubmed ID | |
Authors |
Qi-xin Shi, Liu-kun Yang, Wen-long Shi, Lu Wang, Shi-meng Zhou, Shao-yu Guan, Ming-gao Zhao, Qi Yang |
Abstract |
The G protein-coupled receptor 55 (GPR55) is a novel cannabinoid receptor, whose exact role in anxiety remains unknown. The present study was conducted to explore the possible mechanisms by which GPR55 regulates anxiety and to evaluate the effectiveness of O-1602 in the treatment of anxiety-like symptoms. Mice were exposed to two types of acute stressors: restraint and forced swimming. Anxiety behavior was evaluated using the elevated plus maze and the open field test. We found that O-1602 alleviated anxiety-like behavior in acutely stressed mice. We used lentiviral shRNA to selective ly knockdown GPR55 in the medial orbital cortex and found that knockdown of GPR55 abolished the anxiolytic effect of O-1602. We also used Y-27632, a specific inhibitor of ROCK, and U73122, an inhibitor of PLC, and found that both inhibitors attenuated the effectiveness of O-1602. Western blot analysis revealed that O-1602 downregulated the expression of GluA1 and GluN2A in mice. Taken together, these results suggest that GPR55 plays an important role in anxiety and O-1602 may have therapeutic potential in treating anxiety-like symptoms. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 107 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 26 | 24% |
Student > Ph. D. Student | 12 | 11% |
Student > Master | 12 | 11% |
Researcher | 9 | 8% |
Student > Postgraduate | 5 | 5% |
Other | 13 | 12% |
Unknown | 30 | 28% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 11 | 10% |
Biochemistry, Genetics and Molecular Biology | 9 | 8% |
Agricultural and Biological Sciences | 7 | 7% |
Psychology | 6 | 6% |
Other | 18 | 17% |
Unknown | 41 | 38% |