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Application of a strain rate gradient microfluidic device to von Willebrand's disease screening

Overview of attention for article published in Lab on a Chip - Miniaturisation for Chemistry & Biology, January 2017
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Title
Application of a strain rate gradient microfluidic device to von Willebrand's disease screening
Published in
Lab on a Chip - Miniaturisation for Chemistry & Biology, January 2017
DOI 10.1039/c7lc00498b
Pubmed ID
Authors

Rose J. Brazilek, Francisco J. Tovar-Lopez, Angus K. T. Wong, Huyen Tran, Amanda S. Davis, James D. McFadyen, Zane Kaplan, Sanjeev Chunilal, Shaun P. Jackson, Harshal Nandurkar, Arnan Mitchell, Warwick S. Nesbitt

Abstract

Von Willebrand's disease (VWD) is the most common inherited bleeding disorder caused by either quantitative or qualitative defects of von Willebrand factor (VWF). Current tests for VWD require relatively large blood volumes, have low throughput, are time-consuming, and do not incorporate the physiologically relevant effects of haemodynamic forces. We developed a microfluidic device incorporating micro-contractions that harnesses well-defined haemodynamic strain gradients to initiate platelet aggregation in citrated whole blood. The microchannel architecture has been specifically designed to allow for continuous real-time imaging of platelet aggregation dynamics. Subjects aged ≥18 years with previously diagnosed VWD or who presented for evaluation of a bleeding disorder, where the possible diagnosis included VWD, were tested. Samples were obtained for device characterization as well as for pathology-based testing. Platelet aggregation in the microfluidic device is independent of platelet amplification loops but dependent on low-level platelet activation, GPIb/IX/V and integrin αIIbβ3 engagement. Microfluidic output directly correlates with VWF antigen levels and is able to sensitively detect aggregation defects associated with VWD subtypes. Testing demonstrated a strong correlation with standard clinical laboratory-based tests. Head-to-head comparison with PFA100® demonstrated equivalent, if not improved, sensitivity for screening aggregation defects associated with VWD. This strain rate gradient microfluidic prototype has the potential to be a clinically useful, rapid and high throughput-screening tool for VWD as well as other strain-dependent platelet disorders. In addition, the microfluidic device represents a novel approach to examine the effects of high magnitude/short duration (ms) strain rate gradients on platelet function.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 17%
Student > Bachelor 4 17%
Student > Ph. D. Student 4 17%
Student > Master 3 13%
Professor 2 8%
Other 3 13%
Unknown 4 17%
Readers by discipline Count As %
Engineering 10 42%
Medicine and Dentistry 3 13%
Biochemistry, Genetics and Molecular Biology 2 8%
Chemical Engineering 2 8%
Environmental Science 1 4%
Other 1 4%
Unknown 5 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2018.
All research outputs
#11,839,372
of 13,343,384 outputs
Outputs from Lab on a Chip - Miniaturisation for Chemistry & Biology
#3,487
of 4,016 outputs
Outputs of similar age
#227,220
of 265,987 outputs
Outputs of similar age from Lab on a Chip - Miniaturisation for Chemistry & Biology
#58
of 68 outputs
Altmetric has tracked 13,343,384 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,016 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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