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Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil

Overview of attention for article published in Revista Brasileira de Parasitologia Veterinária, August 2015
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Title
Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil
Published in
Revista Brasileira de Parasitologia Veterinária, August 2015
DOI 10.1590/s1984-29612015051
Pubmed ID
Authors

Jonatas Campos Almeida, Felippe Danyel Cardoso Martins, José Maurício Ferreira Neto, Maíra Moreira dos Santos, João Luis Garcia, Italmar Teodorico Navarro, Emília Kiyomi Kuroda, Roberta Lemos Freire

Abstract

The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a nested-PCR reaction (n-PCR) and sequencing. In the immunofluorescence, 2/24 (8.33%) samples of raw water were positive for Giardia spp.. In n-PCR and sequencing, 2/24 (8.33%) samples of raw water were positive for Giardia spp., and 2/24 (8.33%) samples were positive for Cryptosporidium spp.. The sequencing showed Cryptosporidium parvum and Giardia duodenalis DNA. In raw water, there was moderate correlation among turbidity, color and Cryptosporidium spp. and between turbidity and Giardia spp.. The presence of these protozoans in the water indicates the need for monitoring for water-treatment companies.

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Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 20%
Student > Bachelor 1 20%
Student > Postgraduate 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 20%
Environmental Science 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Immunology and Microbiology 1 20%
Unknown 1 20%