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Site-Specific Recombinases

Overview of attention for book
Cover of 'Site-Specific Recombinases'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Generating Genetically Modified Mice: A Decision Guide
  3. Altmetric Badge
    Chapter 2 Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
  4. Altmetric Badge
    Chapter 3 Building Cre Knockin Rat Lines Using CRISPR/Cas9
  5. Altmetric Badge
    Chapter 4 Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
  6. Altmetric Badge
    Chapter 5 Use of the DICE (Dual Integrase Cassette Exchange) System
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    Chapter 6 Ligand-Controlled Site-Specific Recombination in Zebrafish
  8. Altmetric Badge
    Chapter 7 Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre
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    Chapter 8 Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain
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    Chapter 9 Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
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    Chapter 10 Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X
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    Chapter 11 Nanoparticle-Mediated Recombinase Delivery into Maize
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    Chapter 12 Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
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    Chapter 13 FLPing Genes On and Off in Drosophila
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    Chapter 14 Imaging Neural Architecture in Brainbow Samples
  16. Altmetric Badge
    Chapter 15 Purification and In Vitro Characterization of Zinc Finger Recombinases
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    Chapter 16 Preparing Mate-Paired Illumina Libraries Using Cre Recombinase
  18. Altmetric Badge
    Chapter 17 Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination
  19. Altmetric Badge
    Chapter 18 Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways
  20. Altmetric Badge
    Chapter 19 Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family
  21. Altmetric Badge
    Chapter 20 Production of Minicircle DNA Vectors Using Site-Specific Recombinases
Attention for Chapter 6: Ligand-Controlled Site-Specific Recombination in Zebrafish
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Chapter title
Ligand-Controlled Site-Specific Recombination in Zebrafish
Chapter number 6
Book title
Site-Specific Recombinases
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7169-5_6
Pubmed ID
28815495
Book ISBNs
978-1-4939-7167-1, 978-1-4939-7169-5
Authors

Avinash Chekuru, Veronika Kuscha, Stefan Hans, Michael Brand

Abstract

Cre-mediated site-specific recombination has emerged as an indispensable tool for the precise manipulation of genomes allowing lineage-tracing studies, temporal and spatial misexpressions, and in particular the generation of conditional knockout alleles. Previously, we and others showed that Cre and its ligand-inducible variant CreER(T2) are also highly efficient in the developing and adult zebrafish. The number of Cre driver and effector lines is currently still limited in zebrafish. However, the recent advent of novel genome editing tools such as TALEN and CRISPR/Cas will significantly increase interest in the conditional Cre/lox-technology in this organism. The considerations of basic transgene design and subsequent transgenesis have been addressed elsewhere. Here we outline practical experimental steps for transient functionality tests of CreER(T2) driver and effector constructs. In addition, we introduce detailed protocols to elicit CreER(T2)-mediated recombination in vivo at embryonic as well as adult stages.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 31%
Other 2 15%
Professor > Associate Professor 2 15%
Student > Ph. D. Student 1 8%
Student > Doctoral Student 1 8%
Other 2 15%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 38%
Agricultural and Biological Sciences 4 31%
Neuroscience 2 15%
Medicine and Dentistry 1 8%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2017.
All research outputs
#15,475,586
of 22,997,544 outputs
Outputs from Methods in molecular biology
#5,381
of 13,151 outputs
Outputs of similar age
#257,292
of 421,196 outputs
Outputs of similar age from Methods in molecular biology
#468
of 1,074 outputs
Altmetric has tracked 22,997,544 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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