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Mendeley readers
| Chapter title |
Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
|
|---|---|
| Chapter number | 4 |
| Book title |
Site-Specific Recombinases
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7169-5_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
| Authors |
Eugenia Voziyanova, Rachelle P. Anderson, Yuri Voziyanov |
| Abstract |
Recombinase-mediated cassette exchange, or RMCE, is a genome engineering tool that can be used to swap DNA fragments of interest between two DNA molecules. In a variation of RMCE, called dual RMCE, the exchange of DNA fragments is mediated by two recombinases in contrast to one recombinase in the classic RMCE reaction. Under optimal conditions, the efficiency of dual RMCE can be quite high: up to ~45% of the transfected cells depending on the recombinase pair used to mediate the replacement reaction. Here we describe protocols for preparing for, performing, and optimizing the parameters of dual RMCE. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 38% |
| Student > Ph. D. Student | 1 | 13% |
| Student > Bachelor | 1 | 13% |
| Student > Postgraduate | 1 | 13% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 38% |
| Agricultural and Biological Sciences | 2 | 25% |
| Unknown | 3 | 38% |