| Chapter title |
Nanoparticle-Mediated Recombinase Delivery into Maize
|
|---|---|
| Chapter number | 11 |
| Book title |
Site-Specific Recombinases
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7169-5_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
| Authors |
Susana Martin-Ortigosa, Brian G. Trewyn, Kan Wang |
| Abstract |
We describe a non-DNA-based system for delivering Cre recombinase protein into maize tissue using gold-plated mesoporous silica nanoparticle (Au-MSN). Cre protein is first loaded into the pores of Au-MSNs and then delivered using the biolistic method to immature embryos of a maize line (Lox-corn), which harbors loxP sites flanking a selection and a reporter gene. The release of the Cre recombinase protein inside the plant cell leads to recombination at the loxP sites, eliminating both genes. Visual screening is used to identify recombination events, which can be regenerated to mature and fertile plants. Using the experimental procedures and conditions described here, as high as 20% of bombarded embryos can produce regenerable recombinant callus events. This nanomaterial-mediated, DNA-free methodology has potential to become an effective tool for plant genome editing. |
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|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
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|---|---|---|
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| Researcher | 2 | 15% |
| Student > Doctoral Student | 1 | 8% |
| Librarian | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Other | 1 | 8% |
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| Readers by discipline | Count | As % |
|---|---|---|
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| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Unknown | 8 | 62% |