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Site-Specific Recombinases

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Cover of 'Site-Specific Recombinases'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Generating Genetically Modified Mice: A Decision Guide
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    Chapter 2 Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
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    Chapter 3 Building Cre Knockin Rat Lines Using CRISPR/Cas9
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    Chapter 4 Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
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    Chapter 5 Use of the DICE (Dual Integrase Cassette Exchange) System
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    Chapter 6 Ligand-Controlled Site-Specific Recombination in Zebrafish
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    Chapter 7 Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre
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    Chapter 8 Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain
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    Chapter 9 Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
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    Chapter 10 Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X
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    Chapter 11 Nanoparticle-Mediated Recombinase Delivery into Maize
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    Chapter 12 Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
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    Chapter 13 FLPing Genes On and Off in Drosophila
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    Chapter 14 Imaging Neural Architecture in Brainbow Samples
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    Chapter 15 Purification and In Vitro Characterization of Zinc Finger Recombinases
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    Chapter 16 Preparing Mate-Paired Illumina Libraries Using Cre Recombinase
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    Chapter 17 Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination
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    Chapter 18 Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways
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    Chapter 19 Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family
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    Chapter 20 Production of Minicircle DNA Vectors Using Site-Specific Recombinases
Attention for Chapter 9: Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
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Chapter title
Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
Chapter number 9
Book title
Site-Specific Recombinases
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7169-5_9
Pubmed ID
Book ISBNs
978-1-4939-7167-1, 978-1-4939-7169-5
Authors

Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, David A. Sorrell

Abstract

Site-specific recombinases are important tools for the modification of mammalian genomes. In conjunction with viral vectors, they can be utilized to mediate site-specific gene insertions in animals and in cell lines which are difficult to transfect. Here we describe a method for the generation and analysis of an adenovirus vector supporting a recombinase-mediated cassette exchange reaction and discuss the advantages and limitations of this approach.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 20%
Lecturer 1 20%
Other 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Agricultural and Biological Sciences 2 40%
Unknown 1 20%