Title |
A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice
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Published in |
Nature Communications, June 2014
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DOI | 10.1038/ncomms5091 |
Pubmed ID | |
Authors |
Chen-Chun Pai, Rachel S. Deegan, Lakxmi Subramanian, Csenge Gal, Sovan Sarkar, Elizabeth J. Blaikley, Carol Walker, Lydia Hulme, Eric Bernhard, Sandra Codlin, Jürg Bähler, Robin Allshire, Simon Whitehall, Timothy C. Humphrey |
Abstract |
DNA double-strand break (DSB) repair is a highly regulated process performed predominantly by non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. How these pathways are coordinated in the context of chromatin is unclear. Here we uncover a role for histone H3K36 modification in regulating DSB repair pathway choice in fission yeast. We find Set2-dependent H3K36 methylation reduces chromatin accessibility, reduces resection and promotes NHEJ, while antagonistic Gcn5-dependent H3K36 acetylation increases chromatin accessibility, increases resection and promotes HR. Accordingly, loss of Set2 increases H3K36Ac, chromatin accessibility and resection, while Gcn5 loss results in the opposite phenotypes following DSB induction. Further, H3K36 modification is cell cycle regulated with Set2-dependent H3K36 methylation peaking in G1 when NHEJ occurs, while Gcn5-dependent H3K36 acetylation peaks in S/G2 when HR prevails. These findings support an H3K36 chromatin switch in regulating DSB repair pathway choice. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 3 | 75% |
United Kingdom | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | <1% |
Netherlands | 1 | <1% |
Norway | 1 | <1% |
Austria | 1 | <1% |
Germany | 1 | <1% |
United Kingdom | 1 | <1% |
India | 1 | <1% |
Japan | 1 | <1% |
Canada | 1 | <1% |
Other | 0 | 0% |
Unknown | 197 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 72 | 35% |
Researcher | 44 | 21% |
Student > Master | 16 | 8% |
Professor > Associate Professor | 12 | 6% |
Student > Bachelor | 11 | 5% |
Other | 26 | 13% |
Unknown | 26 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 84 | 41% |
Agricultural and Biological Sciences | 74 | 36% |
Medicine and Dentistry | 10 | 5% |
Neuroscience | 3 | 1% |
Immunology and Microbiology | 2 | <1% |
Other | 5 | 2% |
Unknown | 29 | 14% |