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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Functional characterization and structural modeling of synthetic polyester-degrading hydrolases from Thermomonospora curvata
|
|---|---|
| Published in |
AMB Express, June 2014
|
| DOI | 10.1186/s13568-014-0044-9 |
| Pubmed ID | |
| Authors |
Ren Wei, Thorsten Oeser, Johannes Then, Nancy Kühn, Markus Barth, Juliane Schmidt, Wolfgang Zimmermann |
| Abstract |
Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T. curvata DSM43183 revealed two genes coding for putative polyester hydrolases Tcur1278 and Tcur0390 sharing 61% sequence identity with the T. fusca enzymes. Mature proteins of Tcur1278 and Tcur0390 were cloned and expressed in Escherichia coli TOP10. Tcur1278 and Tcur0390 exhibited an optimal reaction temperature against p-nitrophenyl butyrate at 60°C and 55°C, respectively. The optimal pH for both enzymes was determined at pH 8.5. Tcur1278 retained more than 80% and Tcur0390 less than 10% of their initial activity following incubation for 60 min at 55°C. Tcur0390 showed a higher hydrolytic activity against poly(ε-caprolactone) and polyethylene terephthalate (PET) nanoparticles compared to Tcur1278 at reaction temperatures up to 50°C. At 55°C and 60°C, hydrolytic activity against PET nanoparticles was only detected with Tcur1278. In silico modeling of the polyester hydrolases and docking with a model substrate composed of two repeating units of PET revealed the typical fold of α/β serine hydrolases with an exposed catalytic triad. Molecular dynamics simulations confirmed the superior thermal stability of Tcur1278 considered as the main reason for its higher hydrolytic activity on PET. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 240 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 240 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 37 | 15% |
| Student > Ph. D. Student | 33 | 14% |
| Student > Master | 32 | 13% |
| Researcher | 25 | 10% |
| Student > Doctoral Student | 10 | 4% |
| Other | 30 | 13% |
| Unknown | 73 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 66 | 28% |
| Agricultural and Biological Sciences | 35 | 15% |
| Chemistry | 18 | 8% |
| Environmental Science | 11 | 5% |
| Engineering | 10 | 4% |
| Other | 25 | 10% |
| Unknown | 75 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2022.
All research outputs
#4,593,046
of 22,757,090 outputs
Outputs from AMB Express
#94
of 1,231 outputs
Outputs of similar age
#45,599
of 227,901 outputs
Outputs of similar age from AMB Express
#3
of 21 outputs
Altmetric has tracked 22,757,090 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,231 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,901 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.