Title |
Mouse bladder wall injection.
|
---|---|
Published in |
Journal of Visualized Experiments, July 2011
|
DOI | 10.3791/2523 |
Pubmed ID | |
Authors |
Chi-Ling Fu, Charity A Apelo, Baldemar Torres, Kim H Thai, Michael H Hsieh |
Abstract |
Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. Before starting injections, the surgical area must be cleaned with soap and water and antiseptic solution. Surgical equipment must be sterilized before use and between each animal. Each mouse is placed under inhaled isoflurane anesthesia (2-5% for induction, 1-3% for maintenance) and its bladder exposed by making a midline abdominal incision with scissors. If the bladder is full, it is partially decompressed by gentle squeezing between two fingers. The cell suspension of interest is intramurally injected into the wall of the bladder dome using a 29 or 30 gauge needle and 1 cc or smaller syringe. The wound is then closed using wound clips and the mouse allowed to recover on a warming pad. Bladder wall injection is a delicate microsurgical technique that can be mastered with practice. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 31% |
Student > Doctoral Student | 3 | 12% |
Student > Ph. D. Student | 2 | 8% |
Student > Master | 2 | 8% |
Student > Bachelor | 1 | 4% |
Other | 3 | 12% |
Unknown | 7 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 23% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Agricultural and Biological Sciences | 3 | 12% |
Engineering | 2 | 8% |
Immunology and Microbiology | 2 | 8% |
Other | 3 | 12% |
Unknown | 6 | 23% |