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The Bacterial Nucleoid

Overview of attention for book
Cover of 'The Bacterial Nucleoid'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
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    Chapter 2 Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes
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    Chapter 3 Revealing Sister Chromatid Interactions with the loxP/ Cre Recombination Assay
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    Chapter 4 Transposon Insertion Site Sequencing for Synthetic Lethal Screening
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    Chapter 5 WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites
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    Chapter 6 High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing
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    Chapter 7 Generation and Analysis of Chromosomal Contact Maps of Bacteria
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    Chapter 8 Nucleoid-Associated Proteins: Genome Level Occupancy and Expression Analysis
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    Chapter 9 Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins
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    Chapter 10 A Chromosome Co-Entrapment Assay to Study Topological Protein–DNA Interactions
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    Chapter 11 Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins
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    Chapter 12 Biochemical Analysis of Bacterial Condensins
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    Chapter 13 Exploring Condensins with Magnetic Tweezers
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    Chapter 14 Applications of Magnetic Tweezers to Studies of NAPs
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    Chapter 15 A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data
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    Chapter 16 Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization
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    Chapter 17 Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage
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    Chapter 18 Measuring In Vivo Protein Dynamics Throughout the Cell Cycle Using Microfluidics
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    Chapter 19 Imaging of Bacterial Chromosome Organization by 3D Super-Resolution Microscopy
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    Chapter 20 Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins: The Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
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    Chapter 21 Procedures for Model-Guided Data Analysis of Chromosomal Loci Dynamics at Short Time Scales
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    Chapter 22 Isolation and Characterization of Bacterial Nucleoids in Microfluidic Devices
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    Chapter 23 Modeling Bacterial DNA: Simulation of Self-Avoiding Supercoiled Worm-Like Chains Including Structural Transitions of the Helix
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    Chapter 24 Molecular Dynamics Simulation of Supercoiled, Knotted, and Catenated DNA Molecules, Including Modeling of Action of DNA Gyrase
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    Chapter 25 Erratum to: Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins, the Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
Attention for Chapter 2: Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes
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  • Above-average Attention Score compared to outputs of the same age and source (63rd percentile)

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Chapter title
Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes
Chapter number 2
Book title
Methods in Molecular Biology
Published in
Methods in molecular biology, August 2017
DOI 10.1007/978-1-4939-7098-8_2
Pubmed ID
Book ISBNs
978-1-4939-7097-1, 978-1-4939-7098-8
Authors

Patrick Higgins, N., N. Patrick Higgins

Abstract

DNA gyrase is the only topoisomerase that can catalytically introduce negative supercoils into covalently closed DNA. The enzyme plays a critical role in many phases of DNA biochemistry. There are only a few methods that allow one to measure supercoiling in chromosomal DNA and analyze the role of gyrase in transcription and its interaction with the other three bacterial topoisomerases. Here, we provide molecular tools for measuring supercoil density in the chromosome and for connecting the dots between transcription and DNA topology.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Researcher 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Chemical Engineering 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 August 2017.
All research outputs
#14,952,935
of 22,999,744 outputs
Outputs from Methods in molecular biology
#4,723
of 13,151 outputs
Outputs of similar age
#187,424
of 316,465 outputs
Outputs of similar age from Methods in molecular biology
#30
of 108 outputs
Altmetric has tracked 22,999,744 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,465 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 108 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.