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Chapter title |
A Chromosome Co-Entrapment Assay to Study Topological Protein–DNA Interactions
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Chapter number | 10 |
Book title |
The Bacterial Nucleoid
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7098-8_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7097-1, 978-1-4939-7098-8
|
Authors |
Larissa Wilhelm, Stephan Gruber, Wilhelm, Larissa, Gruber, Stephan |
Abstract |
Chromosome organization, DNA replication, and transcription are only some of the processes relying on dynamic and highly regulated protein-DNA interactions. Here, we describe a biochemical assay to study the molecular details of associations between ring-shaped protein complexes and chromosomes in the context of living cells. Any protein complex embracing chromosomal DNA can be enriched by this method, allowing for the underlying loading mechanisms to be investigated. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 2 | 40% |
Student > Ph. D. Student | 2 | 40% |
Student > Doctoral Student | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 80% |
Nursing and Health Professions | 1 | 20% |